两种方法联合检测梅毒螺旋体抗体的临床应用

Clinical application of joint detection of two methods about Treponema pallidum antibody

目的探讨甲苯胺红不加热血清试验(TRUST)和梅毒螺旋体明胶颗粒凝集试验(TPPA)两种方法同时用于梅毒螺旋体抗体检测的重要性和必要性。方法采用TRUST和TPPA同时检测1 994例临床输血者、手术患者及皮肤科患者的血清梅毒螺旋体抗体,阳性者再进行滴度检查。结果 TRUST阳性22例,阳性率1.1%,TPPA阳性40例,阳性率为2.0%,两种方法同时阳性18例,阳性率为0.9%。结论为尽早查出病原体,防止梅毒进一步传播,同时避免不必要的医患纠纷,对输血前筛查及疑似梅毒患者宜采用TPPA,阳性者再做TRUST检测,既提高阳性检出率,又降低假阳性率。

Objective To study the importance and necessity that syphilis Tolulized red unheated serum test（TRUST）and Treponema pallidum Gelati particle agglutinatio Test（TPPA） for Treponema pallidum antibody simultaneously.Methods The serum Treponema pallidum antibody of the 1 994 clinical blood transfusion,surgery and dermatology patients were tested simultaneously by TRUST and TPPA,and the positive samples were retested for titration.Results 22 cases were positive,positive rate was 1.1% by TRUST.40 cases was positive,the positive rate was 2.0% by TPPA.While 18 cases were positive,the positive rate was 0.9% in both tests.Conclusion Early diagnosis can avoid unnecessary patient disputes,TPPA should be used to detect treponema pallidum antibody for the screening of blood donor and diagnosis of suspected infection,the positive samples were retested by TRUST.Not only to improve the detection rate,but also to reduce the false positive rate.