益肾活血汤对糖尿病性ED大鼠阴茎白膜弹性纤维组织的影响

Effect of YiShenHuoXueTang on albuginea penis fibroelastic tissue in diabetic erectile dysfunction rats′

目的观察益肾活血汤对糖尿病性阴茎勃起功能障碍(ED)大鼠阴茎白膜弹性纤维组织的影响,探讨其治疗糖尿病性ED的可能机制。方法取雄性SD大鼠50只,随机取10只为正常组,余40只以链脲佐菌素诱导建立糖尿病模型。行阿朴吗啡阴茎勃起试验筛选糖尿病性ED模型后,按随机化原则选20只并分为模型组、治疗组各10只。治疗组予益肾活血汤药液17.7 g/(kg.d)灌胃,模型组及正常组予等量生理盐水灌胃。给药4周后,在阴茎海绵体勃起状态下取各组阴茎组织,行维多利亚蓝—丽春红染色,彩色图文系统进行分析,观察阴茎白膜弹性纤维累积光密度(IOD)值。结果治疗组、模型组、正常组阴茎白膜组织中弹性纤维IOD值分别为8.50±2.03、3.96±1.88、9.55±2.67,治疗组明显高于模型组(P<0.01),与正常组接近(P>0.05);模型组显著低于正常组(P<0.01)。结论益肾活血汤可显著提高糖尿病性ED大鼠阴茎白膜弹性纤维数量;此可能为其治疗糖尿病性ED的可能机制。

Objective To investigate the effect of YiShenHuoXueTang on albuginea penis fibroelastic tissue in diabet- ic erectile dysfunction rats＇ in order to reaearch the treating mechanism of YiShenHuoXueTang on diabetic erectile dysfunc- tion. Methods There were 50 male SD rats included in this study. Of all the 50 rats, 10 normal rats were randomly divid- ed into control group and the other 40 rats were induced into diabetic erectile dysfunction model after treated by streptozoto- cin and apomorphine penis erectile test. Twenty diabetic erectile dysfunction rats were chosen randomly and equally divided into two groups, which were diabetic erectile dysfunction group and YiShenHuoXueTang group. Rat s in YiShenHuoXueT- ang group were treated with YiShenHuoXueTang [ 17.7 g/（ kg ~ d） ] by route of gastric perfusion. Rats in diabetic erectile dysfunction group and control group were treated with normal saline in a same dose and route of administration as YiShen- HuoXueTang group. After a drug delivery of 4 weeks, penile tissue of rats in each group was sampled in condition of erec- tion and tested by method of Victoria blue/Ponceau red staining. Integrated optical density （IOD） of elastic fibers in albug- inea penis was detected by means of color image analysis. Results IOD of elastic fibers in albuginea penis in YiShen- HuoXueTang group, diabetic erectile dysfunction group and control group was （ 8.50 ± 2.03 ）, （ 3.96 ± 1.88 ） , （ 9.55 ± 2.67）, respectively. IOD of YiShenI-IuoXueTang group was higher than that of diabetic erectile dysfunction group （ P 〈 O. 01 ） and closed to control group（ P 〉 0.05 ）. IOD of diabetic erectile dysfunction group was less than that of control group （ P 〈 0.01 ）. Conclusions YiShenHuoXueTang increase the amount of the albuginea penis fibroelastic tissue in rat, which indicates that it relats to the improvement of erectile function of diabetic erectile dysfunction rats.