摘要
为了研究10个不同来源的白灵菇品种亲缘关系,采用内转录间隔区(ITS)序列分析技术,系统分析了不同地区白灵菇ITS的多态性.用ClustalX、MEGA4等软件分析结果显示,10个不同来源的白灵菇具有很近的亲缘关系,其中有9个品种的同源性高达98.5%,其可能来自同一品系,而另一个品种与其他9个差异较大,此可能来自其他品系或是一株突变品种.
In order to study the phylogenetic relationship of 10 Pleurotus nebrodensis strains collected from differ ent regions, ITS sequence analysis was used. ITS sequences were amplified using primer ITS1 （5＇-TCCGTAGGT- GAACCTGCGG-3＇） and ITS4（5＇-TCCTCCGCTTATTGATATGC-3＇）, and analyzed using ClustalX and MEGA4 software. The results showed that the ten P. nebrodensis had a very close relationship. The similarity of 9 strains was 98.5% and they might come from the same strain. The other one was a little different from them. It might come from the other strains or be a mutant variety.
出处
《常熟理工学院学报》
2012年第8期61-65,共5页
Journal of Changshu Institute of Technology
基金
常熟市农业科技项目"‘村企挂钩’食用菌设施农业标准化高效栽培技术集成与示范"(CN201001)
苏州市应用基础研究项目"白灵菇种质资源评价及其工厂化栽培良种选育"(SYN201007)
江苏省科技支撑计划项目"食用菌低碳高效生产技术体系建设与示范"(BE2011456)
关键词
白灵菇
rDNA-ITS序列
亲缘关系
Pleurotus nebrodensis
rDNA-ITS sequence
phylogenetic relationship
