p16和p53抑癌基因产物在胰腺癌中表达的研究

Expression of tumor suppressor gene p16 and p53 product in pancreas carcinoma

目的 :探讨抑癌基因p16和p53在胰腺癌中的表达与临床病理的关系及其在胰腺癌发病机制中所起作用。方法 :应用免疫组织化学方法检测 50例胰腺癌p16和p53蛋白的表达水平。结果 :p16蛋白表达阳性率为 4 8% ,组织学Ⅰ级阳性率 (80 % )显著高于Ⅱ级 (50 % )和Ⅲ级 (2 5% ) ;临床Ⅰ期阳性率 (80 % )显著高于Ⅱ期 (38 9% )和Ⅲ +Ⅳ期 (2 9 4 % )。p53蛋白表达阳性率为 54% ,临床Ⅰ期阳性率(2 6 7% )却显著低于Ⅱ期 (6 1 1% )和Ⅲ +Ⅳ期 (70 6 % )。p16发生缺失同时伴有p53基因突变 ,即p16(- )p53(+)共 17例 ,占 34%。结论 :胰腺癌发生中存在p16抑制途径和p53抑制途径改变 ,p16和p53蛋白异常在胰腺癌发生进展中起着重要作用。

Objective:To study the relationship between the expression of tumor suppressor gene p16 and p53 product and clinical pathology in pancreas carcinoma,and its role in tumorous genesis.Methods:p16 and p53 protein level were detected by immunohistochemistry in 50 cases of pancreas carcinoma.Results:The positive rate of p16 protein was 48% and much higher in histological stage Ⅰ (80%) than in stage Ⅱ (50%) and stage Ⅲ (25%, P <0.05),higher in clinical stage Ⅰ (80%) than in stage Ⅱ (38 9%) and stage Ⅲ+Ⅳ (29 4%, P <0.05).The positive rate of p53 protein was 54% and much lower in clinical stage Ⅰ (26 7%) than in stage Ⅱ (61 1%) and stage Ⅲ+Ⅳ (70 6%).Coexistent inactivations of both p16 and p53 was in 17 cases (34%).Conclusions:These two genes function in distinct tumor suppressive pathways and play an important role in the biology of pancreas carcinoma.