摘要
目的:探讨苦参碱对HepG2细胞代谢水平及基因水平的影响。方法:使用不同浓度的苦参碱作用HepG2细胞3天,对PCNA、AFP、N-ar s、e-myc、p53、Rb进行免疫组化检测,对e-myc、p53 mRNA进行原位杂交法检测,对HSGGT进行亲和微柱法检测。结果:苦参碱用药3天后,苦参碱0.8g/L时HepG2细胞AFP和PCNA表达有所减弱,用药1.5g/L时HepG2细胞AFP和PCNA表达为阴性;用药后HepG2细胞相关代谢指标HSGGT明显活性下降,癌基因e-myc表达减弱,抑癌基因p53、Rb表达增强。结论:苦参碱具有抑制人肝癌HepG2细胞增殖的作用,能影响HepG2细胞的增殖代谢及抑制癌基因的增殖。
Objective To study the Effect of matrine on the metabolic level and the level of gene in HepG2 cells, Methods HepG2 cells were cultured about 3 days in different concentrations of matrine, PCNA, AFP, N-ars, e-myc, p53, Rb were detected by immunohistochemistry, e-myc, p53 mRNA by sttu hybridization assay, HSGGT by pro-and micro-column method, Results After 3d,at 0.8g / L of matrine, AFP and PCNA expression weakened at 1.5g / L,AFP and PCNA were negative; The activity of HSGGT decreased obviously, expression of the oncogene of the e-myc decreased,expression of tumor suppressor gene p53 and Rb increased. Conclusion Matrine can affect the proliferation of HepG2 cells metabolism and inhibit the proliferation of cancer genes.
出处
《中国美容医学》
CAS
2012年第09X期22-23,共2页
Chinese Journal of Aesthetic Medicine