脂肪乳剂建立大鼠高脂血症模型的研究

Research of Establishing Hyperlipidemia Model in Rats with Fat Emulsion

目的用两种不同浓度脂肪乳剂按灌胃法建立大鼠高脂血症模型,并对其血脂水平进行评价,为建立合适的高脂血症动物模型提供依据。方法将30只150～180 g健康雄性SD大鼠随机分为正常对照组、低浓度脂肪乳剂组及高浓度脂肪乳剂组,每组10只。分别饲以基础饲料、低浓度配方脂肪乳剂(20%猪油、6%胆固醇、0.2%丙基硫氧嘧啶、2%胆酸钠及10%吐温-80)及高浓度配方脂肪乳剂(30%猪油、10%胆固醇、1%丙基硫氧嘧啶、5%胆酸钠及20%吐温-80)。2周后处死大鼠,测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)水平。结果高浓度脂肪乳剂组血清TC、TG、HDL-C及LDL-C水平与正常对照组及低浓度脂肪乳剂组比较均升高(P<0.05),低浓度脂肪乳剂组血清TC、TG及HDL-C水平与正常对照组比较差异无统计学意义(P>0.05)。结论高浓度脂肪乳剂可以成功建立SD大鼠高脂血症模型,低浓度脂肪乳剂不适合用于建立SD大鼠高脂血症模型。

Objective To compare two ways of establishing hyperlipidemia model in rats with fat emulsion.Methods Thirty male SD rats were randomly divided into three groups,which fed with normal diet（normal control group）,low concentration fat emulsion（low concentration fat emulsion group）,and high concentration fat emulsion（high concentration fat emulsion group）,respectively.All the rats were sacrificed and tested for serum total cholesterol（TC）,triglyceride（TG）,high density lipoprotein-cholesterol（HDL-C）,and low density lipoprotein-cholesterol（LDL-C） in two weeks after establishment.Results The levels of TC,TG,HDL-C,and LDL-C in the high concentration fat emulsion group were significantly higher than those in the normal control group and the low concentration fat emulsion group（P0.05）;the levels of TC,TG,and HDL-C in the low concentration fat emulsion group didn＇t significantly differ from normal control group（P0.05）.Conclusions High concentration fat emulsion can be used to establish hyperlipidemia model in SD rats,low concentration fat emulsion is not suitable for establishing hyperlipidemia model in SD rats.