NaCl和NaHCO_3胁迫对玉米幼苗有机渗透调节物质的影响

Effects of NaCl and NaHCO_3 Stress on Organic Osmotica in Maize(Zea mays L.) Seedlings

[目的]研究NaCl和NaHCO3胁迫对2个基因型玉米(Zea mays L.)幼苗有机渗透调节物质的影响,探讨不同耐性玉米品种对不同盐碱胁迫时间下的耐性机制。[方法]以耐盐碱程度不同的2个玉米品种为试验材料,采用水培法,设置5个浓度梯度(0、25、50、75、100 mmol/L)的NaCl和NaHCO3盐溶液进行胁迫处理,分别于胁迫12、24、36、48 h后采样,测定玉米幼苗期不同胁迫时间下部分有机渗透调节物质含量的变化。[结果]随着胁迫时间的延长,2个玉米品种可溶性糖含量先升高后降低,但是根系的变化幅度普遍高于叶片,可溶性蛋白含量先升高后降低,在36 h盐胁迫下达到最大值;玉米幼苗和根系中脯氨酸含量先降低后升高,在胁迫48 h后达到最大值。[结论]该研究可为作物耐盐碱的生理机制研究提供理论依据。

[Objective] The purpose was to study NaCl and NaHCO3 stress on organic osmotica in two maize seedlings,and discuss different maize varieties＇ resistance mechanism at different stress times.[Method] With hydroponic method,different levels of salinity of two maize varieties was treated with different concentration of NaCl and NaHCO3（0,25,50,75,100 mmol/L）,then some organic osmotica content variation were determined after treating 12、24、36、48 hours.[Result] The results showed that,along with the stress time extended,the content of soluble sugar of the two maize varieties seedings increased at first then reduced,while its change extent in root was higher than that in blade,the change law of soluble protein content was same as soluble sugar,and the content reached maximum after stressing 36 hours;the content of praline in maize seedings and root first decreased then increased,and reached maximum after stressing 48 hours.[Conclusion] The study provides a theoretical basis for researching physiological mechanism of crop resistance to salt.