摘要
[目的]为改进叶黄素的测定方法。[方法]采用丙酮和酒精混合液(体积比1∶1)进行萃取,用分光光度法在不同的萃取时间对胡萝卜、黄槐决明花和柑橘皮中的叶黄素含量进行测定。[结果]胡萝卜萃取12 h时其叶黄素含量达最高(0.315 5 mg/gFW),可见12 h是胡萝卜中叶黄素的最佳萃取时间;黄槐决明花萃取14 h时其叶黄素含量最高(0.818 5 mg/gFW),说明14 h是黄槐花中叶黄素的最佳萃取时间;柑橘皮萃取8 h时其叶黄素含量达最高(0.001 9 mg/gFW),可知8 h是柑橘皮中叶黄素的最佳萃取时间。[结论]为改进叶黄素的测定方法提供了理论依据。
[Objective] To improve determination method for lutein.[Method] By using acetone and alcohol volume ratio(1∶1) to extract,the lutein content in Daucus carrot,Senna surattensis and Citrus reticulata Banco at different extraction time were determined with spectrophotometry method.[Result] The results showed that lutein content in Daucus carrot was up to the highest at 12 h(0.315 5 mg/gFW),and Senna surattensisn at 14 h(0.818 5 mg/gFW),Citrus reticulata Banco at 8 h(0.001 9 mg/gFW).[Conclusion] The study will provide theoretical basis for improving lutein determination method.
出处
《安徽农业科学》
CAS
2012年第33期16099-16101,16112,共4页
Journal of Anhui Agricultural Sciences
基金
广西民族师范学院科研基金资助项目(XYZDa2011001)
关键词
胡萝卜
黄槐决明
柑橘
叶黄素
Daucus carrot
Senna surattensis
Citrus reticulata Banco
Lutein