改进叶黄素测定方法的探讨

Discussion on Improved Determination Methods for Lutein

[目的]为改进叶黄素的测定方法。[方法]采用丙酮和酒精混合液(体积比1∶1)进行萃取,用分光光度法在不同的萃取时间对胡萝卜、黄槐决明花和柑橘皮中的叶黄素含量进行测定。[结果]胡萝卜萃取12 h时其叶黄素含量达最高(0.315 5 mg/gFW),可见12 h是胡萝卜中叶黄素的最佳萃取时间;黄槐决明花萃取14 h时其叶黄素含量最高(0.818 5 mg/gFW),说明14 h是黄槐花中叶黄素的最佳萃取时间;柑橘皮萃取8 h时其叶黄素含量达最高(0.001 9 mg/gFW),可知8 h是柑橘皮中叶黄素的最佳萃取时间。[结论]为改进叶黄素的测定方法提供了理论依据。

[Objective] To improve determination method for lutein.[Method] By using acetone and alcohol volume ratio（1∶1） to extract,the lutein content in Daucus carrot,Senna surattensis and Citrus reticulata Banco at different extraction time were determined with spectrophotometry method.[Result] The results showed that lutein content in Daucus carrot was up to the highest at 12 h（0.315 5 mg/gFW）,and Senna surattensisn at 14 h（0.818 5 mg/gFW）,Citrus reticulata Banco at 8 h（0.001 9 mg/gFW）.[Conclusion] The study will provide theoretical basis for improving lutein determination method.