生育年限对麦冬多糖含量的影响

Effects of Growth Period on the Polysaccharide Content in RADIX OPHIOPOGONIS

[目的]研究不同生育年限的杭麦冬商品块根中多糖含量的相关性规律,确定其品质特征,为麦冬GAP质量控制及资源综合开发利用提供科学依据。[方法]在杭麦冬(3年生)主产区采用原位动态法,按照麦冬栽种后0.5、1、2和3年分别随机采集植株块根样品,将洗净的鲜块根微波杀酶-50℃恒温烘干,采用苯酚-硫酸比色法分别测定各处理麦冬块根的多糖含量,并运用SPSS17.0版统计软件分析不同生育年限的麦冬块根多糖含量的相关性规律。[结果]不同生育年限杭麦冬块根的多糖平均含量为17.68%～34.86%;随着麦冬植株块根生长发育进程增加,麦冬块根的多糖含量呈极显著的正相关增长(P<0.001),其回归方程分别为:生育年限y1=9.126ln(x)+24.58(r2=0.980*),生育时间y2=9.065ln(x)-28.85(r2=0.981)。[结论]麦冬块根生育年限与次生代谢产物多糖的含量密切相关,杭麦冬主产区3年采收是有科学依据的,川麦冬道地主产区由于气候的特殊性,第2年夏秋高温潮湿易导致上年块根大量腐烂,只适宜于1年采收。

[Objective]To study the correlation rules of polysaccharide content in RADIX OPHIOPOGONIS with different growth periods,to determine the characteristic quality,and to provide scientific basis for GAP quality control and for resources rational exploitation and utilization of RADIX OPHIOPOGONIS.[Method] In situ dynamic method was used for the main production area for RADIX OPHIOPOGONIS（3 years old）.Root tuber samples with 0.5,1,2 and 3 years old were randomly collected.The washed fresh root tubers were dried at-50 ℃.Polysaccharide content in each treatment was detected by phenol-vitriolic colorimetry.Correlation rules of polysaccharide content in RADIX OPHIOPOGONIS with different growth periods were analyzed by SPSS17.0 software.[Result] The average content of polysaccharide in RADIX OPHIOPOGONIS with different growth periods was between 17.68% and 34.86%.With the growth of root tuber,polysaccharide content showed extremely significant positive correlation（P0.001）.The regression equations were as follows： y1=9.126ln（x）＋24.58（r2=0.980） and y2=9.065ln（x）-28.85（r2=0.981）.[Conclusion] The content of polysaccharide which was a secondary metabolite was closely related to the growth period.There was scientific basis for harvesting at 3 years old.It was proper to collect one year after plantation,because in the summer and fall of the second year,a large amount of root tubers became rotten due to the high temperature and humidity