各型乙型肝炎患者血清sIL-2R、IFN-γ和IL-l变化的研究

Study on the Level of sIL-2R, IFN-γ and IL-1 in Sera of Various Hepatitis B.

目的:探讨各型乙型肝炎患者血清sIL-2R、IFN-γ和IL-l变化及其临床意义。方法:收集急性肝炎、慢性肝炎、慢性重型肝炙和活动性肝硬变患者血清标本各16份,16例健康人血清标本作为正常对照组,采用酶联免疫吸附试验(ELISA)检测各型乙型肝炎患者血清中sIL-2R和IFN-γ水平,采用MTT法检测各型乙型肝炎患者血清中IL-1水平,并与正常对照组进行比较。结果:急性肝炎患者血清sIL-2R、IFN-γ和IL-1水平均明显升高(P<0.01);慢性肝炎患者血清sIL-2R水平升高(P<0.05),IFN-γ水平无明显变化,IL-1水平稍降低;慢性重型肝炎患者血清sIL-2R和IFN-γ水平升高(P<0.05 and P<0.01 respectiveIy).但IL-1水平降低;活动性肝硬变患者血清sIL-2R和IFN-γ水平明显升高(P<0.01),但IL-1水平无明显变化。结论:血清sIL-2R、IFN-γ和IL-1参与各型乙型肝炎的发病机制,调整血清中细胞因子含量可能有助于乙型肝炎的治疗。

Aim: To study the level of sIL-2R. IFN-T and IL-1 in sera of various hepatitis B. Method: Sera were obtained from 16 patients with acute hepatitis B,chronic hepatitis B.hepatitis gravis B and post hepatitic cirrhosis respectively. sIL-2R and IFN-7 were detected by ELISA. IL-1 were detected by MTT method. 16 healthy donors served as controls. Results; The level of sIL-2R obviously increased in sera of acute hepatitis, chronic hepatitis, hepatitis gravis and posthepatitic cirrhosis compared with controlling phase. The lever of IFN-7 increased in sera of acute hepatitis, hepatitis gravis and posthepatitic cirrhosis, but unchanged in chronic hepatitis. The level of IL-1 increased in had of acute hepatitis, decreased in chronic hepatitis and hepatitic gravis but in cirrhosis had no obvious change. Conclusion: sIL-2R, IFN-7 and IL-1 in sera of various hepatitis may play substantial role in pathogesis of various hepatitis.