血管再狭窄发生过程中血浆组织型纤溶酶原激活物及其抑制剂活性与胶原转换的变化

The Changes of Plasma Tissue Plasminogen Activator and its Inhibitor Activity and Collagen Turnover during Vascular Restenosis Development

为探讨血管再狭窄发生过程中血浆组织型纤溶酶原激活物及其抑制剂活性与胶原转换及骨桥蛋白基因表达的变化 ,采用发色底物法检测组织型纤溶酶原激活物及其抑制剂活性 ;应用Northern印迹观察骨桥蛋白基因表达活性。结果表明 ,大鼠主动脉内皮剥脱后 3天 ,血浆组织型纤溶酶原激活物活性开始升高 ,第 7天达高峰 ,由对照组的 2 96 .1± 12 0 .2IU L上升为 85 6 .2± 195 .3IU L ,之后随内皮剥脱时间的延长虽有所下降 ,但在所观察的时间范围内 ,均显著高于对照水平 (P <0 .0 5 )。羟脯氨酸测定结果显示 ,内皮剥脱后 3天血管壁胶原降解开始增加 ,第 7天达高峰 ,是对照组的 1.6倍。此外 ,大鼠主动脉内皮剥脱诱导了骨桥蛋白基因表达 ,内皮剥脱后 3天其表达活性为对照组的 5倍。提示在血管再狭窄发生过程中 。

Aim To explore the changes of plasma tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI) activity,collagen degradation and osteopontin gene expression during vascular restenosis development. Methods The activities of tPA and PAI were measured by tPA and PAI kits, Northern blotting was used to detect osteopontin gene expression. Results The activity of tPA increased at 3 d after de-endothelization, and peaked at 7 d , the level of tPA increased from 296.1±120.2 IU/L (control group) to 856.2±195.3 IU/L (7 d). The results of hydroxyproline concentration showed that the collagen degradation increased at 3 d and peaked at 7 d after de-endothelialization, about 1.6 times compared with the control. Osteopontin gene expression was remarkably induced by de-endothelization, the peak expression was at 3 d after operation, and was about 5 times comparing with the control, then osteopontin gene expression decreased and returned to the control level at 14 d after operation. Conclusions Osteopontin, collagen turnover and tPA take part in the process of vascular remodeling during vascular restenosis development.