巨噬细胞集落刺激因子对RAW264.7细胞谷胱甘肽过氧化物酶活性及其基因表达的影响

Effect of Macrophage Colony-Stimulating Factor on Glutathione Peroxidase Activity and Gene Expression in RAW 264.7 Cells

为探讨巨噬细胞集落刺激因子抗单核巨噬细胞过氧化损伤的机理 ,以富含巨噬细胞集落刺激因子的L92 9细胞条件培养基作为细胞因子来源 ,采用酶活性测定、反转录聚合酶链反应等技术 ,研究了巨噬细胞集落刺激因子对小鼠巨噬细胞系RAW2 6 4.7细胞谷胱甘肽过氧化物酶活性及mRNA表达的影响。结果发现 ,巨噬细胞集落刺激因子可以提高RAW2 6 4.7细胞硒谷胱甘肽过氧化物酶及非硒谷胱甘肽过氧化物酶活性 ,并增强RAW2 6 4.7细胞血浆谷胱甘肽过氧化物酶mRNA的表达 ,而对RAW2 6 4.

Aim In order to find out the mechanism that macrophage colony-stimulating factor protects monocytes/macrophages from oxidative injury. Methods We used macrophage colong-stimulating factor(MCSF)-riched L929 cell conditioned medium (L929-CM) to investigate the effect of MCSF on glutathione peroxidase (GPx) gene expression in RAW264.7 cells applying enzyme activity determination and reverse transcription polymerase chain reaction (RT-PCR) technique. Results It showed that MCSF could improve selenium-dependent glutathione peroxidase (SeGPx) and non-selenium-dependent glutathione peroxidase (non-SeGPx) activity in RAW264.7 cells. MCSF could also increase plasma glutathione peroxidase (PLGPx) mRNA expression in the cells. However, there was no induction of phosphalipid hydroperoxide glutathione peroxidase (PHGPx) expression by MCSF. Conclusion MCSF might protect monocytes / macrophages from oxidative injury through improving antioxidant enzymes activities and gene expression.