骨髓间充质干细胞移植修复二乙基亚硝胺诱导的肝纤维化(英文)

Effect of bone marrow mesenchymal stem cells transplantation on fibrosis in severe chronic hepatic injury induced by diethylnitrosamine

背景:研究表明间充质干细胞能够改善肝纤维化。目的:观察间充质干细胞对二乙基亚硝胺诱导的大鼠慢性肝损伤模型中对纤维化的作用。方法:20 只雌性 Wistar 大鼠由二乙基亚硝胺诱导致慢性肝损伤模型后,随机等分为 2 组,间充质干细胞组(n=10)在第 4,8,12,16 周时尾静脉注射雄性大鼠来源的间充质干细胞(1×109L-1),模型组注射等量的生理盐水。结果与结论:慢性肝损伤 20 周后,Y 染色体仅能在间充质干细胞组中检测出,说明间充质干细胞被成功移植并存活。MRI显示在间充质干细胞组中有大量的增生性结节。与模型组相比,间充质干细胞组中的结节数、纤维化评分、α-平滑肌肌动蛋白、胶原面积均明显增高(P < 0.05)。表明体循环中的间充质干细胞可以导致其植入肝脏组织,并且在由二乙基亚硝胺诱导的大鼠慢性肝损伤模型中促进了肝纤维化。

BACKGROUND：Many studies have shown that bone marrow mesenchymal stem cells could improve liver fibrosis.OBJECTIVE：To observe the effect of bone marrow mesenchymal stem cells on fibrosis in chronic hepatic injury induced by diethylnitrosamine.METHODS：Twenty female Wistar rats were used to establish the model with chronic hepatic injury induced by diethylnitrosamine,and then the models were randomly divided into two groups.Rats in the mesenchymal stem cells group（n=10） received 1×10 6 /mL male bone marrow mesenchymal stem cells injection through caudal vein at 4,8,12 and 16 weeks,and the rats in the model group received equal volume of saline injection.RESULTS AND CONCLUSION：At 20 weeks after chronic hepatic injury,sex-determining region of Y-chromosome was detected in mesenchymal stem cells group but not in model group.It indicated that bone marrow mesenchymal stem cells were successfully transplanted in mesenchymal stem cells group,and survived.MRI showed that a large number of regenerative nodules could be seen in mesenchymal stem cells group.Comparred with model group,the number of regenerative nodules,fibrosis score,α-smooth muscle actin and percentage of collagen area were increased in mesenchymal stem cells group（P 0.05）.Under our experimental conditions,bone marrow mesenchymal stem cells in systemic circulation leads to their engraftment into liver tissue and promotes fibrosis in rat model of severe chronic liver injury induced by diethylnitrosamine.