促红细胞生成素促进脊髓损伤大鼠移植神经干细胞存活和迁移

Effect of erythropoietin on survival and migration of neural stem cells transplanted into injured spinal cord of rats

背景:如何有效促进移植入脊髓损伤组织内的神经干细胞存活和迁移,是目前神经修复研究的重点。目的:观察促红细胞生成素对脊髓损伤大鼠移植神经干细胞存活、增殖和迁移的影响。方法:将60只SD大鼠随机分为3组,均制备脊髓横断损伤模型。造模7d,神经干细胞移植组和促红细胞生成素组于脊髓损伤处移植BrdU标记的神经干细胞7μL(1×109L-1),脊髓损伤对照组移植DMEM/F12培养基;促红细胞生成素组腹腔内注射促红细胞生成素5000U/kg,1次/d,连续注射7d,其余两组注射等量生理盐水。于细胞移植后8周取损伤脊髓组织。结果与结论:造模2周后,神经干细胞移植组和促红细胞生成素组BBB评分明显高于脊髓损伤对照组(P<0.05),造模4周后,促红细胞生成素组BBB评分明显高于神经干细胞移植组(P<0.05)。免疫荧光染色显示促红细胞生成素组大鼠损伤脊髓组织BrdU阳性细胞数量及迁移距离均大于神经干细胞移植组(P<0.05)。说明促红细胞生成素能促进损伤脊髓组织原位移植的神经干细胞的存活与迁移,加速神经功能修复。

BACKGROUND：How to promote the survival and migration of the neural stem cells transplanted into the injured spinal cord is the focus of the research on nerve repair.OBJECTIVE：To investigate the effect of erythropoietin on the survival,proliferation and migration of neural stem cells transplanted into spinal cord injury rats.METHODS：Sixty Sprague-Dawley rats were randomly divided into three groups：spinal cord injury group,neural stem cells group and erythropoietin group.All the rats were used to make the spinal cord injury model.7 μL（1×10 9 /L） BrdU labeled neural stem cells were transplanted into the spinal cord lesion area of rats in neural stem cells group and erythropoietin group at 7 days after modeling,and DMEM/F12 medium was transplanted into the spinal cord injury group;erythropoietin group was intraperitoneally injected with 5 000 U/kg erythropoietin,once per day and continuously injected for 7 days,the neural stem cells group and spinal cord injury group were injected with normal saline in the same dose.The spinal cord injury tissues were observed at 8 weeks after cell transplantation.RESULTS AND CONCLUSION：At 2 weeks after modeling,the Basso,Beattie and Bresnahan score in neural stem cells group and erythropoietin group was significantly higher than that in the spinal cord injury group（P 0.05）;at 4 weeks after modeling,the Basso,Beattie and Bresnahan score in the erythropoietin group was significantly higher than that in the neural stem cells group（P 0.05）.Immunofluorescence staining showed that the number of BrdU-positive cell and the migration distance of transplanted neural stem cells in the erythropoietin group were significantly higher than those in the neural stem cells group（P 0.05）.The erythropoietin could promote the survival and migration of neural stem cells in situ transplanted into the injured spinal cord,and accelerate nerve function repair.