摘要
目的 :研究空肠弯曲菌 (Cj)的主要外膜蛋白 (momp)基因的分子分型。方法 :采用PCR方法对 163株空肠弯曲菌的主要外膜蛋白基因进行扩增 ,用HindⅢ ,HaeⅢ ,MboⅡ和HhaⅠ四种内切酶消化后进行电泳分析 ,并与热稳定性血清型 (HS)和鞭毛蛋白A基因 (flagellinAgene ,falA)分型方法相比较。结果 :共观察到 8种不同的主要外膜蛋白的限制性片段长度多态性的基因型 ,其中主要外膜蛋白基因 3 (momp 3 )占 61%。Guillain Barr啨综合征(GBS)和腹泻患者所有 3 4株HS 19:Cj(fla) 1菌株均属同一个单一基因型 ,即主要外膜蛋白基因 1(momp 1)。结论 :HS 19:Cj(fla) 1:momp 1菌株是空肠弯曲菌的一个特殊类型 ,与HS 19相关的GBS可能是阐明GBS发病机制最好的模型。
Objective: To study the momecular typing of the major outer membrane protein gene(momp) of Campylobacter jejuni(C.jejuni). Methods: The C.jejuni major outer membrane protein gene was amplified by PCR from a total of 163 strains.After Hind Ⅲ, Hae Ⅲ, Mbo Ⅱ and Hha Ⅰ restriction enzyme digestions,the digest was analyzed by electrophoresis,and then compared with heat stable(HS) serotype and PCR based restriction fragment length polymorphism(RFLP) typing of flagellin A gene (fla A).Results: Eight distinct momp RFLP genotypes were observed.Among all of them,one major type(momp 3) accounted for 61% of all isolates.All 34 HS 19:Cj(fla) 1 strains,including isolates from patients with Guillain Barré syndrome(GBS) and enteritis,belonged to a single genotype(momp 1).Conclusion: The HS 19:Cj(fla) 1:momp 1 strain is distinctive among C.jejuni stains,and HS 19 related GBS may be an excellent model to elucidate the pathogenesis of GBS.
出处
《河南医学研究》
CAS
2000年第2期100-102,共3页
Henan Medical Research
基金
HenanProvincialCommitteeofScienceandTechnology