人肝癌HepG-2细胞对人淋巴管内皮细胞增殖及管状结构形成的影响

The Effect of Human Hepatoma HepG-2 Cells on Proliferation and Tube-like Structure Formation of Human Lymphatic Endothelial Cells

目的探讨人肝癌HepG-2细胞对人淋巴内皮细胞(HLEC)增殖、迁移和管状结构形成的影响。方法采用transwell方法建立HepG-2细胞与HLEC共培养系统;通过绘制细胞生长曲线,观察HepG-2细胞对HLEC增殖的影响;通过细胞迁移实验,观察HepG-2细胞对HLEC迁移的影响;通过基质胶实验,观察HepG-2细胞对HLEC管状结构形成的影响。结果 HepG-2细胞能够显著促进HLEC增殖(P<0.05),迁移(P<0.05)及管状结构形成(P<0.05),呈剂量依赖性。结论 HepG-2细胞促进HLEC增殖、迁移及管状结构形成可能是肿瘤淋巴道转移的机制之一。

Objective To study the effect of Human Hepatoma HepG-2 Cells on Proliferation、migration and tube-like structure formation of Human of Human Lymphatic Endothelial Cells.Methods Co-culture system of human hepatoma HepG-2 cells（HepG-2） and human lymphatic endothelial cells（HLEC） is established by means of transwell chamber;Cell growth curve is used to observe the effect of HepG-2 on the proliferation of HLEC;Migration assay is used to observe the effect of HepG-2 on the migration of HLEC;Matrigel assay is used to observe the effect of HepG-2 on the tube-like structure formation of HLEC.Results HepG-2 cells can promote the proliferation（P0.05）,migration（P0.05） and tube-like structure formation（P0.05）of HLEC.Conclusion HepG-2 cells promote the proliferation,migration and tube-like structure formation of HLEC,which may be one of the mechanisms that causes tumor metastasis.