骨质疏松症大鼠股骨节段性骨缺损边缘区域骨保护素基因的表达

Dynamic expression of osteoprotegerin gene in an osteoporotic rat’s segmental bone defect model

背景:目前国内的报道仅对正常骨质情况下骨缺损的愈合原理及治疗方式的选择进行研究,对骨质疏松情况下发生骨缺损的愈合原理及可能采取的方案几乎空白。目的:观察骨质疏松症大鼠股骨节段性骨缺损模型修复过程中骨保护素在缺损边缘区域的表达情况。方法:SD大鼠建立骨质疏松症模型后于取材前8周,4周,2周,1周,3d建立股骨节段性骨缺损模型,同一天处死大鼠,对股骨节段性骨缺损边缘区域行免疫组化碱性磷酸酶染色,反转录-聚合酶链反应测定骨保护素基因表达。结果与结论:在大鼠骨质疏松性骨缺损修复过程的不同时间,碱性磷酸酶和骨保护素的表达量皆呈先增多后降低的趋势,说明碱性磷酸酶和骨保护素的表达量与骨修复呈正相关,是骨质疏松性节段性骨缺损修复的重要因素。

BACKGROUND：There are many domestic reports regarding the healing principle and treatment choice of bone defects under normal conditions, but few in the case of osteoporosis. OBJECTIVE：To observe the dynamic expression of osteoprotegerin （OPG） gene during restoration of segmental bone defects in osteoporotic rats. METHODS：After osteoporotic models were established in all rats, the models of segmental bone defects could be established at 3 day, and 1, 2, 4, 8 weeks before we extracted the specimens. Then, the expression of OPG and alkaline phosphatase were detected by reverse transcription-PCR and immunohistochemical technique. RESULTS AND CONCLUSION：In different stages of osteoporotic segmental bone defects, the expressions of OPG and alkaline phosphatase were increased in early and middle stages and then decreased. It is indicated that the key factors to repair osteoporotic rat＇s segmental bone defects is the expression of OPG.