移植性鳞癌兔模型的建立及生物学特征

Establishment and the biological characteristics of transplanted squamous cell carcinoma models in rabbits

背景:兔VX2荷瘤目前已广泛应用于全身多个器官肿瘤动物模型的建立,但在耳郭建立鳞癌动物模型至今少有报道。目的:采用2种不同移植瘤方法建立稳定的兔耳VX2移植瘤模型,并对所建肿瘤模型的生物学特性进行观察和比较。方法:将新西兰大白兔分别用瘤细胞悬液注入法和瘤组织块切开植入法移植于兔耳皮下,观察和比较2种方法所建立的模型的肿瘤生长状况、成瘤率和自发转移发生率。结果与结论:瘤细胞悬液注入组和瘤组织块植入组的成瘤率分别为73.3%和50%,差异有显著性意义(P<0.05)。局部淋巴结转移率分别为75%和86%。两种方法建立的兔VX2鳞癌移植瘤模型中,组织呈浸润性生长,瘤细胞核深染,分裂相多见,并呈癌巢排列的符合鳞状细胞癌特征。证实,瘤细胞悬液注入法和瘤组织块切开植入法建立的移植瘤模型均较接近人鳞癌自然生长过程。瘤细胞悬液注入法建立的动物模型,因成瘤率和转移率高,更适合于鳞癌研究。

BACKGROUND：Rabbit VX2 carcinoma has been widely used in building tumor models of multiple body organs, but it is rarely reported to be used for establishing the squamous cell carcinoma model of auricle. OBJECTIVE：To establish the VX2 auricular tumor model in rabbits by two tumor transplantation methods and to investigate its biological characteristics. METHODS：The VX2 cell suspensions were injected in the ears of New-Zealand rabbits by tumor cells suspension injection method and tumor tissue incision implantation method. Then the growth status of the tumors, the tumor-take rates and the metastasis rates of the models were evaluated. RESULTS AND CONCLUSION：The tumor take-rate of tumor cells suspension injection group and tumor tissue incision implantation group was 73.3% and 50%, respectively, and the difference was significant （P 0.05）, metastases rate of lymph node in two groups was 75% and 85.7% respectively. In the rabbit VX2 squamous cell carcinoma transplanted models established by the two methods, the organization showed infiltrative growth, the deeply stained tumor nuclei and division phase were common, and cancer nest arranged cells were in line with the characteristics of the squamous cell carcinoma. It confirmed that the squamous cell carcinoma transplanted model established by tumor cell suspension injection method and tumor tissue incision implantation method was closed to the natural growth process of human squamous cell carcinoma. The animal model established by tumor cell suspension injection method is more suitable for the study of the squamous cell carcinoma due to the high rate of tumor formation and transfer rate.