摘要
以葡萄糖为标准溶液,以3,5-二硝基水杨酸为显色剂,用分光光度法测定海南毛薯干粉中总还原糖含量.结果表明:在波长480 nm处有最大吸收峰,测定回收率最大为101.9%,最小为99.7%,相对误差为±1.9%.实测得毛薯干粉中总还原糖含量为71.08%,毛薯中淀粉含量为63.97%.
Using glucose as standard and DNS as color developing reagent, the content of total reducing saccharide in Hainan Dioscorea esculenta （Lour.） brukill was determined by spectrophotometry. The re- suits showed that the maximum absorption wavelength of the sample was 480nm. The maximum recovery rate was determined as 101.9% , and the minimum was 99.7%. The relative error was ± 1.9%. The content of total reducing saecharide and starch in Hainan Dioscorea esculenta （Lour.） brukill was 71.08% and 63.97% , respectively.
出处
《北京工商大学学报（自然科学版）》
CAS
2012年第5期56-61,共6页
Journal of Beijing Technology and Business University:Natural Science Edition
基金
海口市重点科技计划项目(0000017)
关键词
海南毛薯
还原糖
分光光度法
Hainan Dioscorea esculenta （Lour.） brukill
reducing saccharide
spectrophotometry
