摘要
A rapid and simple high performance liquid chromatography （HPLC） mcthod wiih a UV detection （241 nm） was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard （valdecoxib） were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column （250 mm × 4.6 mm, 5 um） with a mobile phase consisting of acetonitrile：water （50：50, v/v） at flow rate of 1 mL/min. The calibration curve was linear in the range 100 3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor I/X.
A rapid and simple high performance liquid chromatography （HPLC） mcthod wiih a UV detection （241 nm） was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard （valdecoxib） were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column （250 mm × 4.6 mm, 5 um） with a mobile phase consisting of acetonitrile：water （50：50, v/v） at flow rate of 1 mL/min. The calibration curve was linear in the range 100 3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor I/X.
