叶绿体色素提取实验改进的探讨

Discussion on Improvement of Extract Method of Chloroplast Pigment

本试验选用菠菜及天竺葵叶片为试验材料,采用圆形滤纸纸层析法,主要从材料处理、色素提取、色素层析液的选择等方面对叶绿体色素提取与分离实验的最优化进行了探讨。试验结果表明:一般条件下,对于叶绿体色素提取与分离实验,将实验材料放置12小时左右或用开水烫30秒,用95%的酒精加0.3-1g的碳酸钙来提取叶绿体色素,再用93#汽油为层析液来分离色素能达到理想的实验结果。

This study select spinach and geranium leaves as test material, utilize paper chromatography, mainly extracted from the aspect of material handling, Pigment extraction, pigment liquid chromatography to choice chloroplast pigment extraction and separation optimization experiments. The test results show that： the general conditions for the chloroplast pigment extraction and separation experiments, the experimental material for about 12 hours or scalded with boiling water for 30 seconds, with 95% alcohol and 0.3 - I g of calcium carbonate to extract the chloroplast pigments, and then 93 # gasoline for liquid chromatography to separate the pigment to achieve the desired experimental results.