碘化N-正丁基氟哌啶醇抑制兔颈总动脉球囊损伤后血管内膜和平滑肌细胞增殖的作用研究

Effects of N-n-butyl haloperidol iodide on the proliferation of intima and vascular muscle cells of rabbit carotid artery after balloon injury

目的:探讨碘化N-正丁基氟哌啶醇(F2)对兔颈总动脉球囊损伤后内膜及平滑肌细胞增殖的影响。方法:30只新西兰兔随机分为假手术组、模型组、F2高剂量给药组(2mg/kg)、F2中剂量给药组(1mg/kg)、F2低剂量给药组(0.5mg/kg)。模型组及F2各给药组行左侧颈总动脉球囊损伤,各组分别于术后7d取颈总动脉段,常规病理切片,HE染色,免疫组化法测定α-actin、增殖细胞核抗原(PCNA)的表达水平。结果:与假手术组比较,模型组术后7d血管内膜厚度、内膜面积、内膜厚度/中膜厚度、内膜面积/中膜面积、血管壁细胞PCNA表达显著增加(P<0.01),F2剂量依赖地降低上述指标。与假手术组比较,模型组血管壁α-actin阳性染色减少,F2各给药组可增加血管壁平滑肌细胞中α-actin阳性染色率。结论:F2能抑制兔颈总动脉机械损伤后血管内膜和平滑肌细胞的增殖。

AIM： To investigate the effects of N-n-butyl haloperidol iodide（F2） on the proliferation of intima and vascular smooth muscle cells（VSMCs） of the rabbit carotid artery after balloon injury.METHODS： 30 New Zealand rabbits were randomly divided into Sham group,Model group,F2 high-dose treatment group（2 mg/kg）,F2 medium-dose treatment group（1 mg/kg）,F2 low-dose treatment group（0.5 mg/kg）.After 7 days,experimental segments of arteries were harversted and subsequently progressed routine pathological sections,hematoxylin-eosin staining,PCNA expression was measured by immunohistochemical staining.RESULTS：Compared with the sham group,the intimal thickness,intimal area,intimal/medial thickness and intima-to-media area ratio of the Model group significantly increase（P0.01）.F2 caused a dose-dependent reduction in these indicators.F2 dose-dependently decrease the PCNA positive rate of VSMCs in the vascular wall as compared with Model group（P0.01）.CONCLUSION： F2 inhibits the proliferation of intima and VSMCs of rabbit carotid artery after balloon injury.