地塞米松对顺铂诱导的HepG_2细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响

Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3

目的:探讨地塞米松对顺铂诱导HepG2细胞凋亡的影响,并观察Bcl-2和Caspase-3蛋白的表达。方法:HepG2细胞与顺铂及不同浓度的地塞米松共培养,RT-PCR检测Bcl-2、Caspase-3的mRNA表达,Western blot检测Bcl-2、Caspase-3蛋白表达,流式细胞术(FACS)检测各组HepG2细胞的凋亡情况。结果:随着地塞米松浓度的增加,HepG2细胞中Bcl-2蛋白表达量升高,Caspase-3蛋白表达量下降。同时细胞凋亡率降低。结论:地塞米松部分通过Bcl-2途径抑制肝癌细胞的凋亡,而Caspase-3又在调控细胞凋亡中起重要作用。

AIM： To investigate the mechanism of Dexamethasone（DEX） on apoptosis of HepG2 cells induced by Cisplatin（DDP） and the expression of Bcl-2 and Caspase-3.METHODS： HepG2 and DDP were co-cultured,a serials of concentrations DEX were added into it,the expression of mRNA and protein of Bcl-2 and Capase-3 were analyzed separately by RT-PCR and Western blot,and the percentage of cell apoptosis was analyzed by FACS.RESULTS：With DEX increases in concentration,the expression of Bcl-2 was increased and expression of Caspase-3 was decreased correspondently,the rate of apoptosis of HepG2 descends clearly.CONCLUSION： DEX restrains apoptosis of HepG2 cells induced by Cisplatin（DDP） partly through Bcl-2 anti-apoptosis pathways,and Caspase-3 plays an important role in regulating the cell apoptosis.