NQ01单核苷酸多态性和环境因素的交互作用与肝细胞癌的关联研究

Relationship between hepatocellular carcinoma and the interaction between NQO1 polymorphisms and environmental factors

目的研究NQ01基因多态性和环境因素的交互作用与肝细胞癌易感陆的关系。方法采用以医院为基础的病例对照研究方法，运用TaqManMGB荧光定量实时PCR分析方法对病例组400例肝细胞癌患者和对照组400例非肿瘤患者进行NQ01基因C609T位点的基因型分析。以非条件logistic回归模型分析比较各基因型在两组中分布频率的差异，以及基因多态性和环境因素的交互作用。结果NQ01基因C609T位点CC、CT和TT各基因型频率分别为23．75％、50．25％和28．00％，对照组中3种基因型频率分别为37．55％、43．75％和18．25％，差异有统计学意义沪〈0．05）。与CC基因型相比，CT或者TT基因型的个体罹患HCC的风险伽分别为2．106（95％CI：1．137～3．110）和2．564（95％CI：1．357～4．744）。T等位基因携带者患肝细胞癌的危险陛是C等位基因携带者的1．86倍（凹=1．86，95％CI：1．235～2．980）。交互作用分析结果表明NQ01基因多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用，交互作用的OR值分别为2．431、8．359。结论NQ01C609T基因型可能是广西南部地区人群患肝细胞癌的危险因素之一，NQ01基因多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用，能增加罹患肝细胞癌的风险。

Objective To study the relationship between hepatocellular carcinoma （HCC） and the interaction of polymorphisms in the NAD（P）H：quinone oxidoreductase （NQO1） gene with environmental factors using a hospital-based case-control study. Methods Four-hnndred newly diagnosed HCC cases and 400 healthy individuals （non-tumor controls） were enrolled in the study. Demographic information and medical history was obtained by questionnaire. TaqMan minor groove binder real-time PCR was carded out to detect the NQO1 C609T genotype using blood-derived DNA from all study participants. Unconditional logistic regression analysis was carried out to estimate the odds ratios （ORs） and 95% confidence intervals （CIs）. Results The frequencies of NQO1 609 CC, CT and TT genotypes were 23.75%, 50.25% and 28.00% in the HCC group, and 37.55%, 43.75% and 18.25% in the control group. The differences between the HCC and control group reached statistical significance （all P 〈 0.05）. The ORs of NQO1 609 CT and TT genotypes were significantly higher compared to the CC genotype; the adjusted OR（95% CO of CT was 2.106（1.137-3.110） and of TT was 2.564（1.357-4.744）. Individuals carrying the NQO1 609 T allelic gene had a significantly higher risk of HCC than those carrying the C allelic gene; the adjusted OR（95% C/） was 1.86（1.235-2.980）. Interactions were found between hepatitis B virus infection with hepatitis B surface antigen （HBsAg）-positivity and NQOI gene polymorphisms （adjusted OR： 2.431） and history of cancer （adjusted OR： 8.3592）. Conclusion The NQO1 C609T genotype is associated with increased risk of HCC. Interactions between HBsAg-positive infection, history of cancer, and NQO1 gene polymorphisms may contribute to HCC.