摘要
目的研究NQ01基因多态性和环境因素的交互作用与肝细胞癌易感陆的关系。方法采用以医院为基础的病例对照研究方法,运用TaqManMGB荧光定量实时PCR分析方法对病例组400例肝细胞癌患者和对照组400例非肿瘤患者进行NQ01基因C609T位点的基因型分析。以非条件logistic回归模型分析比较各基因型在两组中分布频率的差异,以及基因多态性和环境因素的交互作用。结果NQ01基因C609T位点CC、CT和TT各基因型频率分别为23.75%、50.25%和28.00%,对照组中3种基因型频率分别为37.55%、43.75%和18.25%,差异有统计学意义沪〈0.05)。与CC基因型相比,CT或者TT基因型的个体罹患HCC的风险伽分别为2.106(95%CI:1.137~3.110)和2.564(95%CI:1.357~4.744)。T等位基因携带者患肝细胞癌的危险陛是C等位基因携带者的1.86倍(凹=1.86,95%CI:1.235~2.980)。交互作用分析结果表明NQ01基因多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用,交互作用的OR值分别为2.431、8.359。结论NQ01C609T基因型可能是广西南部地区人群患肝细胞癌的危险因素之一,NQ01基因多态性与肿瘤家族史、HBsAg阳性之间在肝细胞癌发生中存在交互作用,能增加罹患肝细胞癌的风险。
Objective To study the relationship between hepatocellular carcinoma (HCC) and the interaction of polymorphisms in the NAD(P)H:quinone oxidoreductase (NQO1) gene with environmental factors using a hospital-based case-control study. Methods Four-hnndred newly diagnosed HCC cases and 400 healthy individuals (non-tumor controls) were enrolled in the study. Demographic information and medical history was obtained by questionnaire. TaqMan minor groove binder real-time PCR was carded out to detect the NQO1 C609T genotype using blood-derived DNA from all study participants. Unconditional logistic regression analysis was carried out to estimate the odds ratios (ORs) and 95% confidence intervals (CIs). Results The frequencies of NQO1 609 CC, CT and TT genotypes were 23.75%, 50.25% and 28.00% in the HCC group, and 37.55%, 43.75% and 18.25% in the control group. The differences between the HCC and control group reached statistical significance (all P 〈 0.05). The ORs of NQO1 609 CT and TT genotypes were significantly higher compared to the CC genotype; the adjusted OR(95% CO of CT was 2.106(1.137-3.110) and of TT was 2.564(1.357-4.744). Individuals carrying the NQO1 609 T allelic gene had a significantly higher risk of HCC than those carrying the C allelic gene; the adjusted OR(95% C/) was 1.86(1.235-2.980). Interactions were found between hepatitis B virus infection with hepatitis B surface antigen (HBsAg)-positivity and NQOI gene polymorphisms (adjusted OR: 2.431) and history of cancer (adjusted OR: 8.3592). Conclusion The NQO1 C609T genotype is associated with increased risk of HCC. Interactions between HBsAg-positive infection, history of cancer, and NQO1 gene polymorphisms may contribute to HCC.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2012年第11期833-837,共5页
Chinese Journal of Hepatology
基金
国家自然科学青年基金(81001285)I广西教育厅项目(200911MS162)
l吴阶平医学基金(LDWMFI-SY-2011C009)