选择性激活巨噬细胞促进乳腺癌细胞黏附于细胞外基质

Alternatively activated macrophages promote breast cancer cells to adherence on extracellular matrix

背景与目的:在乳腺癌微环境中,肿瘤相关巨噬细胞(tumor associated macrophages,TAMs)促进乳腺癌转移,与病人的预后呈负相关。本研究旨在探讨与TAMs表形和功能相似的选择性激活巨噬细胞(alternatively activated macrophages,M2)是否能促进乳腺癌细胞黏附于细胞外基质(extracellular matrix,ECM),进一步阐明M2促进乳腺癌浸润迁移的作用机制。方法:用密度梯度离心法,从正常成人外周血中分离单个核细胞,体外诱导M2。在乳腺癌细胞与巨噬细胞的无血清共培养体系中,用纤粘连蛋白包被或不包被培养板的底面,模拟乳腺癌微环境,用黏附实验的方法检测M2促进乳腺癌细胞黏附于ECM的作用。结果:在M2的作用下,乳腺癌MDA-MB-231、BT-474、SK-3rd细胞发生黏附于ECM的细胞明显增多;随时间延长,MDA-MB-231细胞黏附于ECM的细胞数明显增多。结论:M2促进乳腺癌细胞黏附于ECM。

Background and purpose： In the microenvironment of breast cancer, tumor associated macrophages （TAMs） promote breast cancer metastasis, and have negative correlation with prognosis of patients. The present study investigated whether the alternatively activated macrophages （M2）, which have the same phenotype and function with TAMs, promoted breast cancer cells adherence on extracellular matrix （ECM）, and further interpreted the mechanism of M2 promoting breast cancer invasion and migration. Methods： Mononuclear cells were isolated from peripheral blood of normal adult by density gradient centrifugation, and induced M2 in vitro. In the coculture system without serum of breast cancer cells and macrophages, coated cultivation plate with or without fibronectin, simulated breast cancer microenvironment, adherence assay was used to detect whether M2 promoted breast cancer cells adherence. Results： In M2 and breast cancer coculture system, the numbers of ECM adherence of MDA-MB-231, BT-474 and SK-3~ breast cancer cells were increased, and the numbers of MDA-MB-231 cells were increased on time dependence. Conclusion： M2 promotes breast cancer ceils adherence on ECM.