hPOT1基因表达与胃癌发生发展的相关性

Correlation of hPOT1 gene expression with development and progression of gastric cancer

目的探讨人类端粒保护蛋白(hPOT1)基因表达与胃癌发生发展的相关性。方法免疫组化SP法和RT-PCR法分别检测100例原发性胃癌患者(A组)、90例胃溃疡患者(B组)的hPOT1蛋白表达和hPOT1单核苷酸多态性(SNP)。结果 A组hPOT1蛋白阳性表达率明显高于B组(86.0%vs.1.1%)(P<0.01)。hPOT1基因表达及基因型与胃癌临床分期、分化程度、浸润程度、淋巴结转移和5年存活率密切相关(P<0.01或P<0.05),而与初复发、年龄、肿瘤大小和组织学类型无关(P>0.05)。与B组相比,A组GG基因型频率以及等位基因G、T频率差异均有统计学意义(P<0.05),而GT、TT分布差异无统计学意义(P>0.05)。结论 hPOT1与胃癌临床分期、分化程度、浸润深度、淋巴结转移及5年存活率等密切相关,并且与GG基因型和等位基因G、T相关联。

Objective To investigate the correlation of gene expression of human protection of telomeres 1 （hPOT1） with development and progression of gastric cancer. Methods The protein expression and single nucleotide polymorphism （ SNP ） of hPOT1 were detected by immunohistochemistry and RT-PCR in 100 cases with primary gastric cancer（group A） and 90 cases with gastric ulcer（group B） , respectively. Results The positive rate of hPOT] protein was higher in group A than that in group B （86.0% vs. 1.1%）（P〈0. 01）. The gene expression and genotype of bPOT1 in group A were correlated with clinical stage, differentiation degree, invasion depth, lymph node metastasis and 5-year survival rate （P〈0. 01 or P〈0. 05）, but were not associated with incipience and relapse, age, tumor size and histology type（P〉0. 05）. Compared with group B, the frequencies of GG genotype and G/T allele were obviously changed（P〈0. 05）, while the distribution difference of GT and TT was not statistically significant in group A（P〉0. 05）. Conclusion hPOT1 is closely correlated with clinical stage,differentiation degree, infiltration depth, lymph node metastasis, 5-year survival rate in gastric cancer and GG genotype,G/T allele.