远志对糖尿病周围神经病变大鼠尾部感觉神经传导速度和坐骨神经醛糖还原酶活性的影响

Effects of Polygala on Caudal sense nerve conduction velocity and aldose reductase activity of sciatic nerve of DPN rats

目的观察远志对糖尿病周围神经病变(DPN)大鼠尾部感觉神经传导速度(SNCV)和坐骨神经醛糖还原酶(AR)活性的作用。方法 36只雄性Wistar大鼠随机分为3组(n=12):正常对照组、DPN模型组和远志治疗组,DPN模型组和远志治疗组大鼠均采用腹腔注射链脲佐菌素的方法建立DPN模型,以尾部SNCV<30 m/s作为DPN成模标准;待DPN模型成功建立后,DPN模型组大鼠不再做任何处理,远志治疗组大鼠给予远志(2.7 g生药/kg/d)灌胃6 w。分别检测各组大鼠血糖、尾部SNCV和坐骨神经AR活性。结果与正常对照组大鼠比较,DPN模型大鼠的血糖、坐骨神经AR活性明显升高,尾部SNCV明显降低(P<0.01);与DPN模型组大鼠比较,远志治疗组大鼠的血糖、坐骨神经AR活性明显降低,尾部SNCV明显升高(P<0.01)。结论远志可明显降低DPN大鼠坐骨神经AR活性、逆转DPN大鼠尾部SNCV减慢,对DPN时周围神经损伤具有明显的改善作用。

Objective To observe the effects of Polygala on caudal sense nerve conduction velocity （SNCV） and aldose reductase （AR） activity of sciatic nerve of diabetic peripheral neuropathy （DPN） rats. Methods 36 male Wistar rats were randomly divided into 3 groups with 12 rats in each group： normal, DPN model and Polygala treatment groups. The rats in DPN model and Polygala treatment groups were established DPN animal model by intraperitoneal injection of streptozotocin, and SNCV 〈 30 m/s was taken as standard. After the DPN rats model were successfully established, the rats in DPN model group were not given more handling, the rats in Polygala treatment group were lavaged with Polygala （2.7 g · kg- 1· d- i ） for 6 weeks. The blood glucose, caudal SNCV and AR activity of sciatic nerve of rats in each group were respectively detected. Results Compared with rats in normal control group, the blood glucose and AR activity of sciatic nerve of rats in DPN model group were increased obviously, caudal SNCV decreased obviously （P 〈 0. 01 ）. Compared with rats in DPN mod- el group, the blood glucose and AR activity of sciatic nerve of rats in Polygala treatment group were decreased obviously, caudal SNCV in- creased obviously （P 〈 0. 01 ）. Conclusions Polygala can obviously decrease the AR activity of sciatic nerve of DPN rats and prevent de- scending of caudal SNCV of DPN rats. So Polygala could improve peripheral nerve iniury apparently during DPN.