电针穴位对非酒精性脂肪肝大鼠脂质代谢的影响

Impact of Electro-acupuncture on Lipid Metalolism in Rats with Non-alcoholic Fatty Liver Disease

目的通过建立非酒精性脂肪肝(NAFLD)大鼠模型探讨针刺对NAFLD脂质代谢的影响。方法将大鼠分为空白组和NAFLD组,NAFLD组大鼠模型建立成功后,再随机分为对照组、电针穴位组和电针非穴组,电针穴位组取"丰隆"、"足三里"、"太冲"、"三阴交"穴,电针非穴组取大鼠尾部中三分之一随机四处,避开尾静脉,"治疗"4周后处死所有动物取材,检测大鼠的血清脂联素(ADP)、瘦素(LEP)、游离脂肪酸(FFA)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、总胆固醇(TC)、甘油三酯(TG)。结果电针"治疗"4周后,电针穴位组与对照组相比较ADP、HDL升高,LEP、LDL、FFA、TC、TG下降(P<0.05),肝组织脂肪性变减轻,电针非穴组与对照组比较变化不明显(P>0.05),肝组织脂肪性变无明显改变。结论电针"丰隆"、"足三里"、"太冲"、"三阴交"等穴位,能升高血清ADP、HDL,降低LEP、LDL、FFA、TC、TG,从而调节脂质代谢,减少脂肪生成,增加脂肪氧化,减轻肝脏脂肪沉积,治疗NAFLD。

Objective To investigate the effect of electroacupuncture on lipid metabolism in rats with nonalcoholic fatty liver disease （NAFLD）. Methods 44 rats were divided into blank group and NAFLD group. The rats in the NAFLD group were randomly selected to be given blank treatment （control） or electroacupuneture stimulation on acupoints （EA）, non-acupoints （ENA）. The EA pointed to ＂Feng long＂, ＂Zu sanli＇, ＂Tai chong＂ and ＂San yinjiao＇. The ENA pointed to four points in the middle one third of the rat tails without touching the veins. Four weeks after the treatment, serum levels of adipinectin （ADP）, leptin （LEP）, free fatty acids （FFA）, high density lipoprotein （HDL）, low density lipoprotein （LDL）, total cholesterol （TC）, triglycerides （TG） of the rats were tested with immunoreagent. Results Compared with controls, the rats with EA treatment had significantly lower levels of serum LEP,LDL,FFA, TC and TG, and higher levels of serum ADP and HDL （P〈 0.05）. The rats with EA treatment also experienced less fatty degeneration in hepatic tissues. Such changes were not observed in the rats with ENA treatment （P〉0.05）. Conclusion EA （Feng long,Zu sanli,Tai ehong,San yiniiao） increases serum ADP and HDL, lowers LEP, LDL, FFA, TC and TG, and thereby reduces lipogenesis and liver fat deposition.