芦笋超雄株的DNA分子标记辅助筛选

Selection of Super-male Plants in Asparagus with DNA Molecular Marker

以芦笋两性株自交S1代雄株为试验材料,首先与优良雌株进行测交,然后利用与芦笋性别决定基因紧密连锁的显性DNA分子标记对苗期各单株进行性别鉴定,推断出超雄株,为芦笋全雄育种提供亲本材料。研究结果表明:显性标记 Asp1-T7 和 Asp1-T7sp特异性好,扩增效果稳定,均能够鉴别芦笋雌、雄性别;一共测验了14 株芦笋两性株自交 S1代的雄株,从中筛选出2株超雄株;对田间测交组合单株显花后性别表型调查的结果表明,DNA 分子标记检测结果与田间实际相符,2株超雄株所对应的组合被确证为全雄组合。

DNA molecular marker assisted selection of super-male plants was conducted for all-male breeding of asparagus.Males in S1 population of andromonoecious plants were test-crossed with elite females. Two DNA molecular markers tightly linked to sex determining gene M,Asp1-T7 and Asp1-T7sp,which showed stable and specific amplification,were used for sex identification of individuals in the progeny at seedling stage.Putative‘supermales’（MM）were differentiated from the normal males（Mm） by the presence or absence of‘all-male’population in the progeny. Two out of 14 male plants test crossed were distinguished as putative‘supermales’. The test result of DNA molecular marker-assisted selection was highly consistent with that of the sexual phenotype in field survey.The corresponding combinations originated from the two super-male plants were also identified as all-male combinations.