肌萎缩侧索硬化患者体内氧化压力的定量分析

Quantitative assay of oxidative stress in patients with amyotrophic lateral sclerosis

目的检测肌萎缩侧索硬化(ALS)患者尿液中8-异前列腺素F_(2a)(8-iso-PGF_(2a))浓度和LDL体外氧化延迟时间的变化,评价ALS患者体内的氧化压力及抗氧化能力情况。方法根据EL Escorial诊断标准选择ALS患者19例为ALS组,同时选择年龄、影响因素相匹配的健康成年男性19例为对照组。通过铜氧化共轭双烯法检测LDL体外氧化延迟时间,酶联免疫吸附法检测尿液中8-iso-PGF_(2a)浓度。结果与对照组比较,ALS组尿液中8-iso-PGF_(2a)浓度虽有升高趋势[(48.28±4.78)ng/mmol肌酐vs(47.25±2.32)ng/mmol肌酐],但差异无统计学意义(P>0.05),而LDL体外氧化延迟时间明显缩短[(59.32±8.65)min vs(83.16±8.37)min],差异有统计学意义(P<0.01)。结论增高的氧化压力与降低的抗氧化能力在ALS的发病机制中具有一定作用。提示有效的抗氧化治疗可能对控制ALS的发展及改善预后有一定的临床意义。

Objective To measure the urinary 8-isoprostaglandin F2a （8-isoPGF2,）and in vitro oxi- dized low density lipoprotein（ox-LDL）lag time in patients with amyotrophic lateral sclerosis （ALS）. Methods Nineteen ALS patients served as an ALS group according to the EL Escorial di- agnosis criteria,and 19 age- and influence factors-matched healthy subjects served as a control group in this study. Their in vitro ox-LDL lag time and urinary 8-isoPGF2a level were measured by copper oxidation-conjugated diene assay and ELISA, respectively. Results No significant differ- ence was found in urinary 8-isoPGF2a level between ALS group and control group although it was higher in ALS group than in control group （48.28 4-4.78 vs 47.25 ±2.32 ng/mmol creatinine, P 〉0. 05）. The in vitro ox-LDL lag time was shorter in ALS group than in control group （59.32±8. 65 vs 83. 16±8.37 min,P〈0.01）. Conclusion Elevated LDL oxidative stress and re- duced 8-isoPGF2a antioxidant ability play a certain role in pathogenesis of ALS, suggesting that ef- fective antioxidant therapy is of certain clinical significance in controlling progression of ALS and improving its prognosis.