乳腺癌组织中DCC MMP-9蛋白表达与影像学特征关系探讨

Correlation of DCC and MMP-9 expression with the ultrasonic appearance in breast cancer

目的检测结直肠癌缺陷基因(DCC)、基质金属蛋白酶-9(MMP-9)蛋白在乳腺浸润性导管癌组织及癌旁组织中的表达,探讨其表达与影像学特征的关系。方法对2010年6月至2011年4月河南省人民医院乳腺外科手术切除的70例乳腺浸润性导管癌标本及癌旁组织标本,采用免疫组织化学法分别检测DCC、MMP-9蛋白表达情况。用彩超观察乳腺癌的影像学特征,测定乳腺浸润性导管癌和乳腺纤维瘤的微血管密度指数(MDI)。结果乳腺癌组织中DCC蛋白表达率为38.6%,低于癌旁乳腺组织(82.9%,P<0.05),乳腺癌组织中MMP-9蛋白阳性表达率(78.6%)明显高于癌旁乳腺组织(17.1%,P<0.05)。超声观察DCC、MMP-9蛋白表达与乳腺癌浸润、转移具有相关性,DCC蛋白阳性表达乳腺癌的MDI与DCC蛋白阴性表达乳腺癌的MDI相比,差异有统计学意义(P<0.05),MMP-9蛋白阳性表达乳腺癌的MDI与MMP-9蛋白阴性表达乳腺癌的MDI相比,差异有统计学意义(P<0.05)。结论 DCC、MMP-9蛋白可能在乳腺癌发生、发展及转移中发挥作用,DCC、MMP-9蛋白与超声影像结合对乳腺癌预后的判断有一定作用。

Objective To investigate the correlation of DCC and MMP-9 expression with the ultrasonic appearance in breast cancer. Methods Seventy samples of breast cancer and surrounding non-turnout breast tissues were resected between June 2010 and April 2011 in the Department of Breast Surgery, the People＇ s Hospital of Henan Province. Expression of DCC and MMP-9 was examined using immunohistochemistry technique. The results were compared with the ultrasonic features of breast cancer. MDI of breast infiltrating ductal carcinoma and breast fibroma was measured. Results The frequency of DCC expression was 82.9% for morphological normal breast tissue and 38.6% for breast cancer respectively （P〈0.05）. The frequency of MMP-9 expression was 17.1% for morphological normal breast tissue and 78.6% for breast cancer respectively （P〈0.05）. DCC and MMP-9 expression correlated with breast cancer invasion and metastasis in uhrasonography. There was significant difference between the MDI of DCC/MMP-9 positive expression and negative expression （P〈0.05）. Conclusion Expression of DCC and MMP-9 may play some roles in occurrence, development and metastasis of breast cancer. Combination of DCC, MMP-9 expression and ultrasonic appearance can be effective to predict the prognosis of breast cancer.