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反相高效液相色谱法测定黄芩漱口液中黄芩苷的含量 被引量:3

Determination of Baicalin in Huangqin Gargles by RP-HPLC
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摘要 目的:建立反相高效液相色谱法测定黄芩漱口液中黄芩苷的含量。方法:采用反相高效液相色谱法,以依利特Krosmasil-C18柱(4.6mm×250mm,5μm)为色谱柱,甲醇–0.2%磷酸溶液(48∶52)为流动相,流速1.0mL·min-1,检测波长280nm,进样量10μL,柱温30℃。结果:在给定的色谱条件下,黄芩苷与相邻组分峰之间分离良好,阴性对照试验无干扰。黄芩苷浓度在20.0~200.0μg·mL-1之间与峰面积呈良好线性关系,回归方程Y=0.4937X+0.3041,r=1.000,平均加样回收率(n=6)为99.0%;RSD=1.73%。结论:本法操作简便,专属性好,结果准确,可作为黄芩漱口液的含量控制方法。 Objective:To establish a method for determination of baicalin in Huangqin Gargles by RP - HPLC. Meth- ods:The RP- HPLC method was set up. A kromasil C18 column(4.6mm × 250mm,51μm) was used as an analyte col- umn, methanol -0.2% phosphoric acid(48:52) was used as mobile phase. The flow rate was at 1. 0mL. min-1. The de- tective wavelength was at 280nm. Injection volume was 10μL, column temperature was 30℃. Results:With this chromato- graphic condition,the baicalin peak in Huangqin Gargles sample chromatogram could be separated with other ingredient peak completely. The calibration curve was linear at a ranges of 20.0 - 200.0μg . mL-1 for baicalin with r = 1. 000. The average recovery( n = 6 ) from samples was 99.0%, RSD was 1.73%. Conclusion : This method is simple, accurateand reproducible, and it can be used for quantitative control method of this preparation.
出处 《中华中医药学刊》 CAS 2012年第11期2384-2386,共3页 Chinese Archives of Traditional Chinese Medicine
关键词 反相高效液相色谱法 黄芩苷 黄芩漱口液 RP - HPLC baicalin Huangqin Gargles
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