线粒体信号通路Cyt-c和Apaf-1表达及艾灸足阳明经穴保护胃黏膜损伤机制的研究

Research on Expression of Cyt-c in Acute Damnification of Gastric and Pre-Moxibustion

目的:通过观察急性胃黏膜损伤后细胞凋亡线粒体信号转导途径中关键物质细胞色素C(Cyt-c)、凋亡激活因子-1(Apaf-1)表达及艾灸"足三里"、"梁门"穴对胃黏膜细胞凋亡的影响,探讨艾灸预处理保护胃黏膜损伤作用的内在机制。方法:24只wister健康大鼠随机分为3组,8只/组,即捆绑对照组、模型对照组和艾灸穴位组。采用Western-blot方法检测大鼠胃黏膜Cyt-c的表达,免疫组化方法检测大鼠胃黏膜细胞凋亡指数(AI)、Apaf-1表达。结果:模型对照组AI、Cyt-c和Apaf-1表达上调,与捆绑对照组相比具有非常显著性差异(P<0.01);艾灸穴位组AI、Cyt-c和Apaf-1表达降低,与模型对照组比较具有显著性或非常显著性差异(P<0.05或P<0.01)。结论:无水乙醇灌胃可引发急性胃黏膜损,经线粒体Cyt-c→Apaf-1信号转导途径启动细胞凋亡程序,而艾灸足阳明胃经"足三里"、"梁门"穴预处理可以抑制细胞凋亡,其保护胃黏膜损伤作用的内在机制可能与下调线粒体凋亡通路Cyt-c和Apaf-1表达有关。

Objective ： To observe the effects of Cytochrome C （ Cyt - c ） and apoptotic protease - activating factor - 1 （ Apaf - 1 ） on apoptosis mitochon - drial pathway and the mechanism of pre - moxibustion at the acu - points of Foot - Yangming meridian in preventing gastric mucosa systemly. Methods：Twenty -four healthy Wister rats were randomly as- signed to binding, model and moxibustion groups. The content of Cyt - c was measured by western - blot method and AI and Apaf - 1 were observed by immunohistochemical method in gastric mucosa. Results ： After treated with ethanol, the AI was increased, the expressions of Cyt - c and Apaf - 1 were increased highly（ P 〈 0.05 or P 〈 0.01 ） compared with those in binding group. While treated with pre - moxibustion at Zusanli and Liangmen, the AI in gastric mucosa was significant- ly decreased,the expressions of Cyt -c and Apaf- 1 were decreased highly（ P 〈 0.05 or P 〈 0.01 ）compared with those in model group. Conclusion：Moxibustion at Zusanli and Liangmen had the protective effects on gastric mucosa of acute damnification in rats. And the mechanism of inhibition apoptosis may be decreasing the contents of Cyt - c and Apaf - 1 so as to suppress the cell apoptosis mitochondrial pathway.