蒙药加味给喜古纳-3汤与透明质酸钠预防术后肠粘连的实验研究

Experimental Study on the Increased Prescription Geixiguna-3 and Sodium Hyaluronate for Preventing Postoperative Intestinal Adhesion

目的:通过对肠粘连大鼠模型预防效果比较研究,证明蒙西医结合防治肠粘连的优越性,并探讨其作用机理。方法:选用40只SD大鼠,随机分为4组:对照组、西药组、蒙药组、蒙西医结合组。全部大鼠制备成肠粘连模型;西药组、蒙西医结合组制模关腹前用西药(1%透明质酸钠凝胶)涂抹在损伤处和切口附近的腹膜上;蒙药组、蒙西医结合组制模前3天和制模后第1天始给予加味给喜古纳-3汤(含生药0.14g/mL)10mL/(kg.d)灌胃;对照组、西药组给予生理盐水10mL/(kg.d)灌胃。制模后观察比较肠鸣音恢复时间和首次排便时间。给药2周后将4组肠粘连大鼠全部处死,对照观察肠粘连程度,并予分级。并采用t检验和χ2检验,秩和检验来比较两组指标的差异。结果:实验观察研究显示蒙西医结合组肠鸣音恢复时间、首次排便时间等观察指标与对照组均有显著差别(P<0.05)。蒙西医结治疗能明显减轻肠粘连程度,粘连程度肉眼分级明显低于对照组、西药组、蒙药组(P<0.05)。结论:蒙西医结合防治SD大鼠肠粘连优于单纯西药和单纯蒙药治疗。

Objective ： By the experimental study to prove the Mongolian - western medicine treatment can prevent post- operative intestinal adhesion and explore the prevention mechanism. Methods ：40 cases of SD rats were prepared into in- testinal adhesion and randomly divided into control group, western medicine treatment group, Mongolian medicine treat- ment group and Mongolian- western medicine treatment group. The control group was given 0.9% Saline[ 10mL/（ kg · d） ]. The western medicine treatment group was given 0. 9% Saline[ 10mL/（kg · d） ] and Sodium Hyaluronate on the surface of wound. The Mongolian medicine treatment group was given increased prescription Geixiguna- 3 （0.14g/mL of crude drug） [ 10mL/（ kg · d） ]. The Mongolian - western medicine treatment group was given increased prescription Geix- iguna-3（0.14g/mL of crude drug）[ 10mL/（kg · d）] and Sodium Hyalnronate on the surface of wound. The recovery time of bowel sounds and first defecation time were observed. After 2 weeks the rats were killed and compared the condi- tion of intestinal adhesion. Results ： The recovery time of bowel sounds as well as first defecation time of Mongolian - west- ern medicine treatment group were less than those in control group （ P 〈 0.05 ）. Mongolian - western medicine treatment can significantly reduce the extent of postoperative intestinal adhesion（ P 〈 0.05 ）. Conclusions ： Mongolian - western med- icine therapy is beneficial to preventing postoperative intestinal adhesion.