红阳猕猴桃遗传转化体系的建立

Establishment of a Genetic Transformation System of Hongyang Kiwifruit

以红阳猕猴桃叶片和茎段为实验材料,通过优化培养基,建立了一个高效的红阳猕猴桃遗传转化体系,利用农杆菌介导转化法把猕猴桃ACO基因(ACC oxidase gene)的反义链导入红阳猕猴桃,共获得47株潮霉素抗性植株.随机挑选其中14株进行PCR鉴定,其中9株成功扩增到了目的条带,初步统计阳性植株为64.3%左右.此研究为利用分子遗传基因工程手段培育优质延熟保鲜的转基因猕猴桃新品种打下了基础.

The leaves and stems of Hongyang Kiwifruit plants were used as explants.A stable and efficient regeneration system was established for gene transformation by optimizing the culture medium.By using A-grobacterium-mediated method,the antisense expression vector of ACO gene was successfully used to trans-form Hongyang Kiwifruit,and positive transgenic plants were obtained.A total of 47 hygromycin resistant plants were obtained,and 14 of them were chosen randomly for PCR analysis.Nine of the analyzed plants were shown to be positive for the ACO gene insert（64.3%）.The research provides a technology platform for kiwifruit improvement through biotechnology.