大鼠怀孕及哺乳期铅暴露致子鼠海马c-JUN表达的变化

Expresstion Changes of c-jun in Offspring Rat Hippocampus from their Mother Exposured Lead during Pregnancy and Lactation

通过观察大鼠怀孕期及哺乳期铅摄入致子鼠海马回c-jun的变化。用RT-PCR的方法分别检测大鼠怀孕期铅暴露后子鼠和母鼠哺乳期铅暴露后子鼠(子鼠出生早期经乳汁染铅后)脑海马组织的c-jun的变化。结果:大鼠怀孕早期和晚期铅暴露,子鼠出生2 d时海马的c-jun的表达明显受到抑制,出生后经哺乳低剂量铅暴露子鼠c-jun的表达受到明显抑制,而高剂量暴露的却增加了c-jun的表达,达对照组的3倍。子鼠出生4 d海马的c-jun的表达与对照组比较略高,且与出生2 d相比均有所增加。而出生后经哺乳高剂量铅暴露子鼠c-jun的表达依然明显高于对照组,达对照组的4倍。子鼠出生10 d海马的c-jun的表达较4 d时下降,但怀孕早期高剂量暴露组和晚期各暴露剂量均高于对照组。而出生后经哺乳暴露鼠c-jun的表达呈增多趋势,其中高剂量铅暴露子鼠c-jun的表达依然明显高于对照组,达对照组的近10倍。子鼠生后20 d时海马的c-jun的表达均显著增加,各剂量组呈现剂量效应,即随剂量增加表达增多,但表达量均低于对照组。结论:大鼠不同的铅暴露时间和强度对子鼠脑海马c-jun的表达变化不同,同时建立了RT-PCR的检测方法并在毒理学实验中进行了初步应用。

To obtained expresstion changes of c-jun of Hippocampus m onsprmg ~aL~ mother exposured lead during Pregnancy and lactation. Reverse transcription-polymerase chain reac- tion （RT-PCR） was undertaken to examine this expresstion changes. Results： c-jun expression was significantly suppressed in the hippocampus of Offsprings born in 2 days which during early and late pregnant mother rats lead exposured. After birth through breast-feeding in a low dosage lead expo- sured, c-jun expression was also significantly suppressed in the hippocampus, while high dosesage of exposure in breast-feeding offspring but increased expression of c-jun, reaching 3 times than the con- trol group. In 4 days later, c-jun expression was higher than 2 days and also higher than control groups. After 10 days c-jun expression was lower than 4 days. But c-jun expression amount in coun-trol group rats lower than the offspring of high dosage lead exposured during pregnant early time and all dosage lead exposured during pregnant late time. C-jun expressed was increased in offspmg which exposed lead after birth through breasffeeding among them the high-dosage lead exposure offspring C- jun expression remained significantly higher, reaching nearly 10 times than control group. C-inn ex- pressed was significant increased and have dose effect in offsprng in 20 days, but this increase lower than control group. Conclusion：the C-jun expressed in offspring with the lead exposured time and in- tention of mother. We set up the method of Reverse transcription-polymerase chain reaction （ RT- PCR） and applied in toxicology experiment.