六种生物药物分子的高分辨太赫兹光谱研究

High-Resolution THz Spectra of Six Biological and Pharmaceutical Materials

利用太赫兹时域光谱技术,在77～295K之间,分别测量了两种异黄酮(金雀异黄酮和鹰嘴豆芽素A)、两种瘦肉精(盐酸克伦特罗和沙丁胺醇)和两种人参皂苷(Rg2和Rg3)等三类药物生物分子随温度变化的低频振动光谱,发现结构和功能相似的同类样品在室温环境下的太赫兹光谱均存在明显的不同。当温度从295K冷却到77K,测量样品的特征峰增加,鹰嘴豆芽素A可以观察到13个高分辨的特征吸收峰;并伴随线宽变窄,可以为1.9×10-5 mol用量的人参皂苷20-(R)-Rg3提供较准确的吸收峰。得到的高分辨太赫兹指纹谱为快速、无损鉴定该类药物生物分子提供一种可靠的新方法。

The temperature dependent vibrational spectra of three biological and pharmaceutical sets,genistein and biochanin A,clenbuterol hydrochloride and salbutamol,as well as ginseng R2 and R3,in the range of 0.2~4.5 THz（6.6~150.0 cm-1） are presented over the temperature range from 295 to 77 K.Although there are only some minor difference in their molecular structures,the spectra of two samples in every group are quite differences in both absorption band positions and their relative intensities,and display strong linewidth narrowing and frequency blue-shift with cooling.Au 77 K,13 highly resolved spectral features for biochanin A were obtained and the sensitivity of the experiment allows detection of amounts as small as 1.9×10^-5 mol of ginseng R3.Such high-resolution THz fingerprint spectra provide a rapid,nondestructive and reliable method for the identification of these pharmaceutical settings molecules.