高脂组和正常组兔肝脏组织中Zn,Fe,Cu,Mn,Cr,Pb和Cd含量测定和对比分析

Measurement and Comparison of Zn,Fe,Cu,Mn,Cr,Pb and Cd in Rabbit Liver of High-Adipose Group and Normal Group

讨论实验性动脉粥样硬化兔肝脏组织中Zn,Fe,Cu,Mn,Cr,Pb和Cd含量的变化。采用高脂饮食复制兔动脉粥样硬化模型,获取肝脏,用HNO3-H2O2混合液微波消解样品,采用原子吸收光谱法测定肝脏组织中Zn,Fe,Cu,Mn,Cr,Pb和Cd的含量。正常组肝脏组织Zn,Fe,Cu,Mn,Cr,Pb和Cd含量分别为137.6,362.3 14.39,9.599mg.kg-1和159.3,355.0,290.0μg.kg-1,高脂组肝脏组织Zn,Fe,Cu,Mn,Cr,Pb和Cd含量分别为86.09,277.1,11.07,5.366mg.kg-1和115.2,286.0,210.5μg.kg-1。高脂兔肝脏Zn,Fe,Cu,Mn,Cr,Pb和Cd含量降低,可能与食物摄取和脂蛋白代谢异常有关。

The objective of the present paper is to explore the experimental changes in the content of Zn,Fe,Cu,Mn,Cr,Pb and Cd in white rabbit liver with atherosclerosis disease.The method is to reproduce white rabbit liver with atherosclerosis disease by a high-fat diet,and then measure the Zn,Fe,Cu,Mn,Cr,Pb and Cd content with atomic absorption spectrometry.Results show that the Zn,Fe,Cu,Mn,Cr,Pb and Cd contents in the fodders of the normal group are 137.6,362.3,14.39,9.599 mg·kg^-1 and 159.3,355.0,290.0 μg·kg^-1,and 86.09,277.1,11.07,5.366 mg·kg^-1 and 115.2,286.0,210.5 μg·kg^-1,in high-adipose group.It was concluded that Zn,Fe,Cu,Mn,Cr,Pb and Cd contents in white rabbit liver decline,which may have something to do with the intake of foods and the abnormalities of lipoprotein metabolism.