高分辨率熔解曲线分析技术——单核苷酸多态性(SNP)及突变研究的新工具
摘要
随着分子生物学技术的发展,在完成了人类基因组计划之后,人类已进入了后基因组时代。其中很重要的一部分,便是进行基因组多样性的研究。基因组多样性的变异,可以表现为:突变、插入、缺失、串联重复数目的变异,以及单核苷酸多态性(SingleNucleo.tidePolymorphism,SNP)。其中的SNP在基因组中分布十分广泛,是基因组多样性的主要表现。
出处
《医学新知》
CAS
2012年第5期362-364,367,共4页
New Medicine
参考文献20
-
1Distefano G,Caruso M,La Malfa S,et al. High Resolution Melting Analysis Is a More Sensitive and Effective Alternative to Gel - Based Platforms in Analysis of SSR -An Example in Citrus[ J]. PLoS One, 2012,7(8) : e44202.
-
2Slinger R, Bellfoy D, Desjardins M, et al. High - solution melting as- say for the detection of gyrA mutations causing qainolone resistance in Salmonella enteric serovars Typhi anti Paratyphi [ J]. Diagn Mierohiol Infect Dis ,2007,57:455-458.
-
3Kwong A, Ng EK, Wong CL, et al. Identification of BRCA1/2 Found- er Mutations in Southern Chinese Breast Cancer Patients Using GeneSequencing and High Resolution DNA Melting Analysis [ J ]. PLoS One ,2012,7 : e43994.
-
4Wittwer C T, Reed G H, Gundrt C N, et al. High - resolution genoty- ping by amplicon melting analysis using LCGreen [ J ]. Clin Chem, 2003,49 : 853-860.
-
5Jancik S, Drabek J, Berkovcova J, et al. A comparison of Direct se- quencing, Pyrosequencing, High resolution melting analysis, TheraS- creen DxS,and the K- ras StripAssay for detecting KRAS mutations in non small cell lung carcinomas. [ J ]. J Exp Clin Cancer Res, 2012,31:79.
-
6Luming Zhou, Alexander N M, Josuhua G V, et al. Closed - tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye[ J]. Clin Chem ,2004,50 : 1331-1333.
-
7Herrmann M G, Durtschi J D, Bromley L K, et al. Amplieon DNA mehing analysis for mutation scanning and genotyping:crossplatform comparison of instruments and dyes [ J ]. Clin Chem, 2006,52 : 494- 503.
-
8Herrmann M G, Durtschi J D, Bromley L K, et al. Instrument comparison for heterozygote scanning of single and double heterozygotes:a correction and extension of Herrmannetal [ J ]. Clin Chem,2007,53 : 150-152.
-
9Herrmann M G, Durtschi J D, Wittwer C T, et al. Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping [ J ]. Clin Chem, 2007,53 : 1544 -1548.
-
10Tu' Nguyen - Dumont, Florence Le Calvez - Kelm, Nathalie Forey, et al. Description and validation of high - throughput simultaneous genotyping and mutation scanning by high - resolution melting curve analysis[ J]. Human Mutation ,2009,30:884-885.
-
1何江,易海华,周鹏程,崔北金,吴炳耀,孙立新.高分辨率熔解曲线分析技术在物种鉴定与分型中的应用[J].中国媒介生物学及控制杂志,2016,27(2):206-208. 被引量:3
-
2何江,周鹏程,崔北金,吴炳耀,胡双双,杨庆贵,陈峰,孙立新.高分辨率熔解曲线分析技术在鼠形动物鉴定中的应用[J].中国国境卫生检疫杂志,2016,39(2):86-90.
-
3Johann Greilhuber,孙海汐.植物基因组多样性[J].国外科技新书评介,2013(3):7-7.
-
4吴春旭.高分辨率熔解曲线分析技术——分子遗传分析的新曙光[J].生物技术世界,2008(2):44-46. 被引量:2
-
5王保成(编译).圈养老虎的基因组多样性[J].科学新闻,2008(11):7-7.
-
6李宏宇,盛晓华.SNP与基因组多样性[J].今日科技,2001(1):35-36.
-
7倪慧芳,刘次全.人类基因组多样性保护与伦理学和法律问题初探[J].Zoological Research,1998,19(5):405-409. 被引量:4
-
8丛晓霏,丁君,常亚青.虾夷马粪海胆溶菌酶基因SNP标记的开发及多态性分析[J].中国农业科技导报,2012,14(5):136-144.
-
9许丽萍,杜若甫.人类基因组多样性计划的新动态[J].中华医学遗传学杂志,1999,16(2):108-110. 被引量:15
-
10大熊猫“晶晶”基因组完成测序[J].科学中国人,2010(3):42-42.