摘要
目的利用6-羟基多巴胺(6-OHDA)损毁的大鼠帕金森病(PD)模型,研究钙阻断剂伊拉地平对神经元的保护作用。方法大鼠给予伊拉地平1周后,纹状体内注射6-OHDA造模,然后继续给药2周。用圆柱体试验和旋转试验评价损毁程度,用酪氨酸羟化酶(TH)免疫组化染色计数多巴胺神经元数量。结果行为学测试表明,伊拉地平预给药大鼠运动功能障碍有所改善。免疫组化染色显示,用药组黑质多巴胺能神经元较模型组显著增多,提示伊拉地平预给药具有神经保护作用。另1组动物在损毁后给药则没有明显改善。结论伊拉地平有希望成为具有神经保护作用的新型抗PD药物。
Objective To explore the neuroprotective effects of calcium blocker isradipine in a 6-OHDA lesioned rat model of Parkinson＇s disease（PD）.Methods Rats were pretreated with isradipine for one week before they received a 6-OHDA injection into the striatum,then they were treated with isradipine for two more weeks.Cylinder test and apomorphine-induced rotation test were performed for evaluating the severity of lesion.Immunohistochemistry staining of tyrosine hydroxylase（TH）was used for evaluating the survival of dopaminergic neurons.Results Behavior tests showed that locomotion impairment was improved by pretreatment of isradipine.TH staining indicated elevated number of DA neurons in the substantia nigra.However,rats treated with isradipine after lesion did not show any protective effects.Conclusion Pretreatment with isradipine before lesion protected 6-OHDA-induced neurotoxicity,indicating a novel strategy for the therapy of PD.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2012年第5期581-584,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
