中国人群1号染色体长臂散发性结直肠癌相关抑癌基因筛选

Tumor suppressor gene screening on the long arm of chromosome 1 in Chinese patients with spo-radic colorectal cancer

目的筛选与中国人群的结直肠癌发生相关的抑癌基因。方法构建包含1号染色体长臂高频杂合缺失区域的基因芯片，对19例结直肠癌标本进行表达谱分析，并与临床病理特征加以统计学分析，筛选该区域与结直肠癌相关的未知抑癌基因。结果通过数据库检索，我们挑选了25个基因进行相关基因筛选。发现CSRPl、LMODl、PPPlRl2B和CFHL34个基因表达显著下调，分别在15、16、16、16例肿瘤组织中表达下调，其中下调比例超过2倍的例数分别为11、11、14、10例。统计学分析发现这4个基因表达与临床病理特征之间无相关。通过生物信息学分析，推测CSRPl基因可能是与结直肠癌发生相关的抑癌基因。结论表达谱芯片以及生物信息学分析表明CSRPl基因可能是结直肠癌发生相关的抑癌基因。

Objective To screen out the potential tumor suppressor gene （TSG） on D1 S413- D1 S2622 in Chinese patients with sporadic colorectal cancer. Methods On the basis of our previous stud- y, we chose 25 genes located in the D1S413-D1S2622 region and presented a microarray-based high throughput screening approach in 19 sporadic colorectal cancer cases to identify candidate eolorectal cancer- related tumor suppressor genes. The relationship between expression levels of candidate genes and the clini- copathological data was analyzed. Results According to the microarray-based high throughput screening, we found 4 significantly down-expressed genes, including CSRP1, LMOD1, PPP1R12B and CFHL3, which were found to be down-regulated in 15, 16, 16, and 16out of the 19 cases, respectively. Each of them was down-regulated over 2-fold in 11, 11, 14 and 10 samples, respectively. There was no significant association between expression levels of CSRP1, LMOD1, PPP1R12B, CFHI3 and the clinieopathological data. By database searching, CSRP1 was hypothesized to be a eolorectal cancer related tumor suppressor gene. Conclusion Through microarray-based high-throughput screening and subsequent database search- ing, we present the evidence that CSRP1 might be involved in the progression of colorectal cancer.