骨髓间充质干细胞对大鼠急性缺血再灌注肾损伤的修复作用

Repair of acute ischemia-reperfusion renal injury by bone marrow mesenchymai stem cells trans- plantation in rats

目的观察骨髓间充质干细胞（BMSCs）移植对大鼠缺血再灌注（IR）急性肾损伤（AKI）的修复作用。方法将50只SD大鼠随机分为3组，干细胞移植组（IR＋BMSCs组）18只，生理盐水注射组（IR组）18只，正常组（Sham组）14只。分离及培养大鼠BMSCs到第3代，建立大鼠缺血再灌注AKI模型，24h后移植干细胞；检测血清及尿肌酐值，并以细胞核增殖抗原（Ki-67）进行肾脏免疫组织化学观察。结果BMSCs培养后，得到纯度高、生长状态良好的第3代（P3）BMSCs。造模后第4天，细胞治疗组血清肌酐浓度为（37．34±4．22）μmol／L，尿肌酐水平为（22．75±6．23）μmol，而生理盐水注射组血清肌酐浓度为（238．34±32．42）μmol／L，尿肌酐水平为（7．68±1．03）μmol，两组比较差异有统计学意义（P〈0．01）；组织切片学观察，与对照组比较，IR＋BMSCs组肾小管结构改善及上皮细胞数量增多、刷状缘部分恢复，Millar法评分IR＋BMSCs组为0．799±0．023，IR组为2．798±0．055，两组比较差异有统计学意义（P〈0．01）。以Ki-67进行免疫组织化学IR＋BMSCs组有大量增殖抗原阳性表达。结论BMSCs对缺血再灌注性AKI有良好的修复作用。

Objective To observe the repair of ischemia-reperfusion （IR） acute kidney injury （AKI） by bone marrow mesenehymal stem cells （BMSCs） transplantation in rats. Methods Fifty SD rats were randomly divided into three groups： stem cell transplantation group （IR ＋ BMSCs group） （n = 18）, saline injection group （IR group） （n = 18）, and the normal group （Sham） （n = 14）. Rat bone marrow BMSCs were separated and cultured to passage 3, and administrated 24 h after establishment of rat IR AKI model. The serum and urine creatinine levels were determined, renal immunohistochemistry with Ki-67 was done. Results Bone marrow BMSCs were obtained with high purity and good growth state of the passage 3 （P3）. Four days post-modeling, the serum and urine creatinine levels in IR ＋ BMSCs group was （37. 34 ± 4.22） and （22.75±6.23）μmol/L, and those were （238.34±32.42） and （7.68±1.03）μmol/L in IR group, respectively （P 〈 0. 01 ）. Morphologically, tubular structure was improved, the number of epi- thelial ceils was increase and the brush border was partially restored in IR ＋ BMSCs group as compared with tR group. The Millar scores in IR ＋ BMSCs group and IR group were 0. 799±0. 023 and 2. 798 ±0. 055 seperately （P 〈 0. 01 ）. A large number of positive expression of Ki-67 was seen in IR ＋ BMSGs group. Conclusion There was good repair of IR AKI by bone marrow BMSCs transplantation in rats.