三种鼠胰腺癌动物模型的建立

The establishment of three forms mouse pancreatic cancer animal models

目的探索建立3种不同类型的鼠胰腺癌动物模型，基于核磁共振代谢组学技术发现鼠及人类胰腺癌的生物标志物。方法模型1：人胰腺癌细胞株2×10^6个／只接种于裸鼠皮下，建立裸鼠皮下植入人胰腺癌细胞动物模型；模型2：取成模的皮下荷瘤鼠肿瘤组织，剪成1mm^3小粒后植入裸鼠胰体尾处被膜下，建立裸鼠胰腺原位植癌动物模型；模型3：将致癌剂7，12-二甲基苯并蒽（DMBA）混悬液以10mg／100g注入仓鼠胰腺体部被膜下，术组乙醇饮水，建立DMBA胰腺原位诱癌动物模型。结果裸鼠皮下植入人胰腺癌细胞动物模型组成瘤率为100％；裸鼠胰腺原位植癌动物模型组成瘤率为90％；DMBA胰腺原位诱癌动物模型成瘤率为43％（13／30）。结论建立了成熟可靠的各种鼠胰腺癌模型。

Objective Establishment of three kinds of pancreatic cancer nude mice animal mod- els. NMR-based Metabouomic approach was proposed to distinguish mice and human pancreatic cancer＇ s biomakers. Methods Inoculate human pancreatic cancer cell line 2 × 10^6/only in the HYPO of root fore- limb to establish the subcutaneously transplanted tumor animal model. Plant pancreatic cancer tissue 1 mm3 wad into the body of pancreas in nude mice to establish the orthotopic transplantation tumor animal model. Embed DMBA 10 mg/100 g into somatic part of the pancreas in C57BL/6 Inbred Mice to establish the pan- creatic orthotopic induce tumor animal model. Results The achievement ratio of the subcutaneously trans- planted tumor animal model is 100% ;The achievement ratio of the orthotopic transplantation tumor animal model is 90% ; The achievement ratio of the pancreatic induced tumor animal model by 7,12-dimethyl- benzanthracene（ DMBA） is 43%. Conclusion The stable and reliable pancreatic tumor animal models in mouse were established successfully.