蛇床子素的抗焦虑作用研究

Research of the anxiolytic effect of osthol

目的运用行为药理学手段观察蛇床子素(osthol,Ost)对小鼠的抗焦虑作用。方法采用高架十字迷路实验、明暗穿箱实验、新型食物消耗实验和包埋实验观察蛇床子素对焦虑模型小鼠的影响。结果蛇床子素3.50 mg/kg组能明显增加小鼠在高架十字迷路开臂的次数与时间,与溶媒对照组比较,差异有统计学意义(P<0.05);蛇床子素7.00 mg/kg可使小鼠在明箱停留时间和穿箱次数增加,增强小鼠对明箱的探究兴趣;蛇床子素(3.50、7.00、14.00 mg/kg)可显著增加小鼠5 min内的新型食物消耗量;蛇床子素(14.00、28.00 mg/kg)能明显减少被小鼠包埋的玻璃球个数,抑制小鼠的包埋活动。4个实验中,蛇床子素都表现出显著的抗焦虑效果。结论蛇床子素对焦虑模型小鼠具有良好的抗焦虑作用。

Objective To observe the anxiolytic effects of osthol on mice by pharmacology, M.ethoda The anxiolytic effects of osthol on anxiety model mice were observed by using elevated cross road maze experiment, light and dark box going ex-periment, new food consumption experiment and embedding experiment. Results The frequency and time that the mice opened their arms in elevated cross road maze experiment were significantly increased in osthol of 3,50 mg/kg group. It had significant difference （P 〈 0.05） compared with the menstruum control group; the time of staying in light box and going through the other box increased in osthol of 7.00 mg/kg group, and the light box exploring interest of the mice increased. The new food consumption of the mice in 5 minutes significantly increased in osthol （3.50, 7.00, 14.00 mg/kg） group; the number of the embedded glass bails by mice significantly reduced in osthol （14.00, 28.00 mg/kg） group, so it restricted the mice from embedding. In the four experiments, the osthol had shown significant anxiolytic effects. Conclusion Osthol has a good anxiolytic effect on anxiety model mice.