酶解栀子苷制备苷元的正交试验研究

Orthogonal test for preparing genipin with enzymolyzed geniposide

目的:正交试验优化酶解栀子苷制备苷元工艺。方法:以栀子苷元浓度为评价指标,以加酶量、酶解液pH、酶解时间、酶解温度为考察因素,通过正交试验选取最佳酶解工艺。结果:最佳水解条件如下:选用一定纯度的栀子苷粗品,在摇床转速100 r·min-1,栀子苷质量浓度40 g·L-1下,按酶苷质量比1∶1加β-葡萄糖苷酶,酶解液pH 4.5,酶解20 h,酶解温度50℃,栀子苷元转化率可达85.8%。结论:该工艺稳定、可行,可为栀子苷酶解制备苷元提供一定理论依据。

Objective： To optimize the process for preparing genipin with enzymolyzed geniposide by an orthogonal experiment. Method ： The optimal enzymolysis process was selected by an orthogonal experiment, with the concentration of geniposide as the index as well as enzyme quantity, pH of enzymolysis solution, enzymolysis time and enzymolysis temperature as considerations. Result： The optimal hydrolysis conditions were as follow： rough genipin samples at the concentration of 40 g·L ^-1were selected and shook on a ta- ble concentrator at a speed of 100 r · min^-1 , added with β-glucosidase-geniposide 1：1 （weight proportion）, with pH of enzymolysis solution at 4.5, hydrolyzation temperature at 50 ℃ , the conversion rate of genipin could reach 85, 8%. Conclusion： The process is so stable and feasible that it can provide theoretical basis for the preparation of genipin with enzymolyzed geniposide.