藤黄中藤黄酸2种异构体的HPLC含量测定

Content determination of two isomers containd in Garcinia hanburyi by HPLC

目的:建立2种藤黄酸异构体的高效液相含量测定方法。方法:采用色谱柱SunFire(Waters)C8(2.1 mm×150mm,3.5μm),流动相为乙腈-甲醇-0.3%三氟乙酸溶液(36∶37∶27),检测波长360 nm,流速0.3 mL·min-1,柱温28℃。结果:R-藤黄酸,S-藤黄酸线性关系方程分别为Y=2.87×106X-2.24×105,r=0.999 9;Y=3.31×106X-1.44×105,r=0.999 9。平均回收率分别为100.0%,100.9%;RSD分别为2.1%,2.5%(n=6)。藤黄药材供试品中R-藤黄酸和S-藤黄酸的平均质量分数为30.06%,21.45%。结论:本方法简便、稳定,可用于藤黄中藤黄酸2种异构体的鉴定与含量测定。

Objective： To establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC. Method： Chromatographic column of SunFire （Waters） Cs （ 2. 1 mm × 150 mm,3.5 μm） was adopted, with acetonitrile-methanol-O. 3 % trifluoroacetic acid （36：37：27 ）as the mobile phase. The detection wavelength was 360 nm, the flow rate was 0. 3 mL ·min^- 1 , and the column temperature was 28℃. Result： The linear regression equation of r-gambogic acid was Y = 2.87 × 10^6X -2.24 ×10^5, r =0. 999 9. The linear regression equation of S-gambogic acid was Y=3.31 ×10^6X - 1.44 × 10^5 ,r =0. 999 9. The average recoveries were 100. 0% and 100. 9% ,with RSD being 2. 1% and 2.5% （n =6） ,respectivley. The average contents of two gambogic acid in G. hattburyi were 30. 06% and 21.45%, respectively. Conclusion： The method was so convenient and stable that it can be used for identification and content determination of two isomers containd in G. hanburyi.