穴位注射黄芪注射液对肿瘤患者放化疗后白细胞减少症及细胞免疫功能的影响

Acupoint injection of astragalus membranaceus injection on patients with tumor leukopenia after radiotherapy and chemotherapy and its influence on cellular immune function

目的观察足三里穴位注射黄芪注射液对肿瘤患者放化疗后白细胞减少及细胞免疫功能的影响。方法按随机数字表法将2010年3月至2011年8月湖北省襄阳市中心医院60例恶性肿瘤放化疗后的患者随机分为两组30例，治疗组采用足三里穴位注射黄芪注射液治疗，对照组口服利血生、维生素B4、鲨肝醇治疗。两组患者分别于治疗前后查外周血白细胞，并经直接免疫荧光染色法及流式细胞仪检测外周血T淋巴细胞总数及其亚群变化。结果①临床疗效：治疗组总有效率为77．5％，对照组为52．5％，两组总有效率比较，差异有统计学意义（X2=8．027，P〈0．05）。②白细胞计数及外周血T淋巴细胞亚群比较，治疗组白细胞计数为（3．56～4．21）×10^9／L，对照组为（2．89～3．39）×10^9／L，两组比较差异有统计学意义（t=2．435，P〈0．05）治疗组CD3＋、CD4＋、CD4＋／CD8＋[（62±1．63）％、（43±0．80）％、（1．48±0．23）]较同组治疗前[（48±1．25）％、（3l±0．74）％、（0．93±0．12）]明显升高（P〈0．05或0．01），对照组治疗前后无明显改变（P〉0．05）；两组治疗后比较有显著性差异（P〈0．05或0．01）。结论足三里穴位注射黄芪注射液可调节患者细胞免疫功能，治疗放化疗后白细胞减少症。

Objective To observe the effect of Zusanli acupoint injection of Astragalus Membranaceus Injection on patients with tumor leukopenia after radiotherapy and chemotherapy and its influence on cellular immune function. Methods According to clinical case of random table, 60 cases of malignant tumor patients in Hubei province Xiangyang city hospital from March 2010 to August 2011 were randomly divided into two groups of 30 patients in each group. The treatment group was treated with Zusanli acupoint injection of Astragalus Injection therapy, while the control group was taken Leucogen, vitaminB4, batyl alcohol orally. Venous blood and peripheral white blood cell were taken in both groups before and after treatment. Direct immunofluorescence staining and flow cytometry were used for the detection of peripheral blood T lymphocyte total number and its subsets. Results （1） clinical efficacy： Total effectiveness was 77.5% in the treatment group, and 52.5% the control group, the difference was statistically significant between the two groups （Z2=8.027, P〈0.05 ）. Comparison of the white blood cell count and T lymphocyte subsets of peripheral blood： the white blood cell count was （3.56-4.21） x10^9/L in the treatment group, and （2.89-3.39） x 10^9/in the control group, there was significant difference between two groups （t=2.435, P〈0.05）; there was significant elevation of CD3 ＋, CD4＋, CD4＋/CD8＋ [ （62±1.63） %, （43± 0.80） %, （1.48 ±0.23） in the treatment group after the treatment comparing with （48±1.25） %, （31±0.74） %, （0.93± 0.12） before the treatment （P〈 0.05 or 0.01）, while there was no such changes in the control group. Conclusion Zusanli acupoint injection of Astragalus Injection may modulate the cellular immune function of patients with cancer. It is effective in the treatment of leukopenia after radiotherapy and chemotherapy.