HPLC法测定烈香杜鹃中苄基丙酮的含量

Determination of benzylacetone in the rhododendron by HPLC

目的建立烈香杜鹃中苄基丙酮的含量测定方法。方法采用高效液相色谱法，LunaC，s（250mrnX4．6mm，5μm）色谱柱，柱温30°C，流动相为乙腈-水（64：36），流速：1ml／min，检测波长为220hi／1。结果在上述色谱条件下，苄基丙酮在17．08～170．8μg／ml范围内与峰面积呈良好的线性关系（R=0．9998），平均回收率为99．81％，RSD=1．46％（n=6）。结论该方法简便、快速、准确，可作为烈香杜鹃挥发油及其制剂的质量控制方法。

Objective To establish a HPLC method for determination of benzylacetone in the rhododendron. Methods The HPLC analysis was carried out on Luna C18（250 mmx4.6 mm, 5 μm） at 30°C temperature. The mobile phase was acetonitrile-water （64 ： 36） , the flow rate was 1 ml/min, and the detection wavelength was 220 nm. Results The linear range of benzylacetone was 17.08- 170.8μg/ml （R=0.9998） , the average recovery of this method was 99.81%, and the RSD was 1.46%. Conclusion The method is convenient, quickly and accurate, can be used for the quality control of rhododendron oil and their preparations.