基于尼龙微孔薄膜的微流控芯片的制作及对葡萄糖的显色响应

Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose

以尼龙微孔薄膜为基质材料,采用光刻法将正性紫外光刻胶转移到薄膜上形成目标微通道图形,制作了一种新型薄膜微流控芯片.对尼龙微孔薄膜芯片的制作工艺进行了优化和改进,使其制作时间控制在1 h内,且制作步骤大为简化,可实现快速、批量地制作尼龙薄膜芯片.在该芯片上进一步固定葡萄糖氧化酶和辣根过氧化物酶后,利用二步酶促化学反应开展了葡萄糖的显色响应研究.结果表明,所制作的尼龙薄膜芯片对不同浓度的葡萄糖均能产生明显的颜色响应,具有较宽的浓度响应范围和良好的重复性,并可用于实际样品中葡萄糖的显色响应研究.

A new type of nylon membrane-based microfluidic chips was fabricated by transferring the positive photoresist onto the thin membrane material via photolithography. It was found that the fabrication period can be controlled in 1 h with substantive production, and the whole fabrication processes was simplified and improved. Meanwhile, by fixing horseradish peroxidase and glucose oxidase on the membrane chips, glucose samples were assayed at different concentrations based on the two-steps enzymatic reactions. Additionally, the obviously changed color corresponding to the gradient concentration of glucose shows that the new type membrane chips could be used extensively in practice in the future.