月季花抗氧化活性成分研究

Studies of Chemical Constituents from Rosa chinensis Jacq.

以活性追踪为指导分离和纯化了月季花中具有1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除活性的成分.从月季花中分离得到了10个黄酮类化合物,采用光谱法对其结构进行了表征,并对它们的自由基清除活性进行了评价.结果表明,化合物1为新化合物,化合物1,2,4和9具有较强的清除DPPH自由基的活性.

In order to separate and purify the chemical constituents from Rosa chinensis Jacq., which have DPPH· scavenging capacity, dried Rosa chinensis flowers were extracted three times with 95% ethanol under reflux. This extract was then fractionated by column chromatography over silica gel, MCI gel, Sephadex LH-20, etc. A new compound[quercetin 3-O-（2＂,6＂-digalloyl）-β-D-glucoside, 1] and nine known compounds （2-10） were isolated from Rosa chinensis Jacq. and identified using spectroscopic methods. Their antioxidant activity was assayed using DPPH· experiments. The results indicated that compounds 1, 2, 4 and 9 had good antioxidant activity.